The Investigator Argus X-12 QS Kit uses QIAGEN's fast-cycling PCR technology for the direct, simultaneous amplification of 12 X-chromosomal markers, D21S11 and Amelogenin. The amplification of the 12 X-chromosomal markers (see Table 1 and 2), with heightened discrimination power, meets the needs for complex kinship and paternity testing and helps fulfill the demands of complicated deficiency cases involving at least one female. The 12 X-chromosomal markers are clustered into four linkage groups (three markers per group; Table 1), and thus each set of three markers is handled as a haplotype for genotyping.
Table 1. Linkage groups
||DXS8378 – DS10135 – DXS10148
||DXS7132 – DXS10074 – DXS10079
||HPRTB – DXS10101 – DXS10103
||DXS7423 – DXS10134 – DXS10146
The innovative Quality Sensors provides additional information on the sample quality and PCR success beyond the profile of your sample. With the Quality Sensor (QS) being amplified as an amplification control, it is possible to determine:
- A successful PCR run
- A failed PCR run
- Lack of DNA in your sample
This additional information will help you analyze your data, and guides you with respect to further sample processing. It will also help you reduce unnecessary PCR re-runs, saving you time and money.
The additional autosomal marker D12S11 acts as alignment marker. Now, you have the possibility to align your X-chromosomal profile from the Investigator Argus X-12 QS Kit with a profile of any other autosomal STR kit from the expanded CODIS or ESS market, to minimize the risk of sample mix-ups.