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RNAprotect Tissue Reagent
For immediate stabilization of RNA in tissues
RNAprotect Tissue Reagent immediately stabilizes RNA in tissue samples to preserve the gene expression profile. It is a storage solution that permeates harvested tissues rapidly to avoid freezing samples in liquid nitrogen. RNAprotect Tissue Reagent is supplied in 50 ml or 250 ml bottles. For stabilization of DNA, RNA, and protein in tissue samples, Allprotect Tissue Reagent can be used. The following kits are compatible with the RNAprotect Tissue Reagent: RNeasy Plus Kits, RNeasy Kits, miRNeasy Kits, and AllPrep Kits. Buy Products
The RNAprotect Tissue Reagent is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAGEN’s former RNA Stabilization Reagent, was manufactured and offered under license from Ambion, Inc., the owner of the RNAlater® trade mark. Following the expiration of that license and corresponding patents, QIAGEN’s new product name for the identical formulation is RNAprotect Tissue Reagent. Product Details
Performance
RNAprotect Tissue Reagent stabilizes and protects the RNA expression pattern of samples during harvest and storage (see figure "RNAprotect Reagent prevents degradation of mRNA in tissues"). Immediate protection with RNAprotect technology ensures that downstream analyses truly reflect the expression profile of the intact tissues. Samples can be archived without risk of RNA degradation, even after multiple freeze–thaw cycles. RNeasy purified RNA from RNAprotect stabilized samples stored under a wide variety of conditions is ideal for downstream processes such as northern blotting and RT-PCR.
Principle
Once a biological sample is harvested, its RNA becomes extremely unstable. The innovative, aqueous RNAprotect Tissue Reagent quickly permeates tissues resulting in immediate stabilization of the RNA and preservation of the RNA expression pattern. Gene induction or down-regulation triggered by sample manipulation is also prevented, allowing accurate analysis of gene expression.
Procedure
For RNA protection and sample storage, tissue samples are submerged in an appropriate volume of RNAprotect Tissue Reagent immediately after harvesting. RNAprotect Tissue Tubes provide premeasured volumes of RNAprotect Tissue Reagent in reclosable tubes for convenient handling and sample storage. The reagent preserves RNA for up to 1 day at 37°C, 7 days at 18–25°C, or 4 weeks at 2–8°C, allowing processing, transportation, storage, and shipping of samples without liquid nitrogen or dry ice. Alternatively, the samples can also be placed at –20°C or –80°C for archival storage.
Applications
Animal and human tissues stabilized in RNAprotect Tissue Reagent can be efficiently disrupted using QIAGEN instruments for sample disruption, and used with QIAGEN kits for:
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