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PAXgene Blood ccfDNA Tubes (100)

For collection of whole blood samples and stabilization of ccfDNA

Features

  • Single-tube collection, stabilization, transport and storage
  • Immediate stabilization of circulating, cell-free DNA (ccfDNA)
  • Sample transport and storage at room temperature for up to 10 days
  • Standardized preanalytical processing of samples

Useful Resources

PAXgene Blood ccfDNA Tube (RUO) Handbook Purification of genomic DNA from the PAXgene® Blood ccfDNA Tube using the QIAamp® DNA Blood Mini Kit - (EN) PAXgene Blood ccfDNA System Brochure
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PAXgene Blood ccfDNA Tubes (100)

Cat. No. / ID: 768115

100 blood collection tubes (10 ml). To be used in conjunction with the QIAamp MinElute ccfDNA Kit, the QIAamp Circulating Nucleic Acid Kit (50) or the QIAsymphony PAXgene Blood ccfDNA Kit (192)
 R$5,646.00
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

✓ 24/7 automatic processing of online orders

✓ Knowledgeable and professional Product & Technical Support

✓ Fast and reliable (re)-ordering

Product Details


The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. When the tube is used in conjunction with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, high-quality ccfDNA can be isolated for use in downstream analytical assays.

Performance

PAXgene Blood ccfDNA Tubes simplify the collection and processing of whole blood for subsequent purification of circulating, cell-free DNA (ccfDNA). The tube additive stabilizes blood cells (see " PAXgene Blood ccfDNA stabilization helps prevent hemolysis allowing distinct separation of plasma" and " PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions") and blocks apoptosis (see " PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA"). Consequently, using PAXgene Blood ccfDNA Tubes for whole blood collection, storage and transport helps prevent the release of intracellular DNA into plasma (see " PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma"). ccfDNA levels are stable for up to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C prior to processing, so blood samples can be transported and stored to process plasma later.
Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes and processed with the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAGEN QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit, yields high-quality ccfDNA for downstream analytical assays, including but not limited to PCR-based assays and next-generation sequencing (NGS).

See figures
BC_0716_PX_ccfDNA_002BC_0716_PX_ccfDNA_002LG_0314_PX_ccfDNA_005LG_0314_PX_ccfDNA_005G_0384_PX_ccfDNA_003G_0384_PX_ccfDNA_003ID_0560_PX_ccfDNA_001ID_0560_PX_ccfDNA_001

Principle

The PAXgene Blood ccfDNA Tube is a plastic, closed, evacuated tube for the collection, anticoagulation, transport and storage of human whole blood samples and the stabilization of ccfDNA. The tube contains an additive that anticoagulates blood and stabilizes blood cells via a non-crosslinking stabilization solution. This helps prevent the release of intracellular DNA into the plasma and maintains constant ccfDNA levels during sample storage before processing.

Procedure

The PAXgene Blood ccfDNA Tube allows collection of 10 ml whole blood into a closed, evacuated system. Using standard venipuncture technique, blood is collected into an evacuated tube that contains 1.5 ml cell stabilization additive, resulting in a ratio of 0.15 ml additive per 1 ml blood. Isolation of ccfDNA from plasma is carried out using the QIAsymphony PAXgene Blood ccfDNA Kit, the QIAamp MinElute ccfDNA Kit or the QIAamp Circulating Nucleic Acid Kit.

Applications

ccfDNA purified from plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be used in a wide range of downstream applications, including:

  • PCR, including digital, multiplex and quantitative real-time PCR
  • Pharmacogenomic studies
  • SNP genotyping
  • Next-generation sequencing

Supporting data and figures

PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.

Whole blood was stored in EDTA tubes or PAXgene Blood ccfDNA Tubes. ccfDNA was extracted from the plasma immediately following blood collection (t0) or after 6 days storage (t6) at room temperature. Eluate (1 µl) was analyzed using the Agilent High Sensitivity DNA Kit. Plasma from EDTA tubes showed an increase in apoptotic gDNA fragments, whereas plasma from PAXgene Blood ccfDNA Tubes showed a ccfDNA profile comparable to day 0.

PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.
PAXgene Blood ccfDNA stabilization reagent helps prevent release of gDNA into plasma.
PAXgene Blood ccfDNA stabilization reagent helps prevent hemolysis, allowing clear distinction between plasma and cell fractions.
PAXgene Blood ccfDNA stabilization helps prevent release of gDNA into plasma during storage and transport.
PAXgene Blood ccfDNA stabilization minimizes hemolysis compared to alternative solutions.
PAXgene Blood ccfDNA stabilization helps prevent apoptosis and fragmentation of gDNA in whole blood.

Specifications

FeaturesSpecifications
Tube size16 x 100 mm
StabilizationUp to 10 days at temperatures up to 25°C, 7 days at temperatures up to 30°C, or 3 days at temperatures up to 37°C. Note: Do not store blood-filled tubes below 2°C.
Draw volume10 ml
Label typePaper
Quantity100 tubes/case
Closure type/colorPearl white BD Hemogard/Blue stopper
Additive/concentrationAdditive 1.5 ml

Resources

Brochures & Guides (2)
PAXgene Blood ccfDNA System Brochure
PDF (1MB)
PAXgene Blood ccfDNA System Transport Concept
PDF (1MB)
Scientific Posters (10)
EGFR Mutation Analysis – Performance Evaluation of Blood Collection Tubes for ccfDNA Stabilization
PDF (7MB)
Ullius et al. 2019

 

Precision and Robustness of the PAXgene Blood ccfDNA Workflow
PDF (6MB)
Krenz et al. 2019

 

Comparison of Hemolysis in Circulating Cell-free DNA Stabilization Tubes
PDF (6MB)
Hallemariam et al. 2019

 

A New Blood Collection Tube and Automated Extraction Method for Stabilization and Analysis of ccfDNA
PDF (3MB)
Ullius et al., 2015

 

Integrated Workflow for Blood Collection, ccfDNA Stabilization and Extraction for Non-invasive Prenatal Testing
PDF (7MB)
Ullius et al., 2016

 

Performance Evaluation of a New Integrated Blood Collection and Sample Preparation System for the Stabilization and Extraction of Circulating Cell-free DNA (ccfDNA)
PDF (5MB)
Ullius et al.

 

Multimodal Analysis of Circulating Tumor Cell RNA, Circulating Cell-Free DNA and Genomic DNA from a Single Blood Sample Collected into the PAXgene Blood ccfDNA Tube
PDF (3MB)
Babayan et al. 2019

 

A Shortened, Automated Workflow for Processing Plasma Directly From Primary Blood Collection Tubes Reduces Turnaround Time and Risk of Sample Mix-up in ccfDNA Based Applications
PDF (4MB)
Voss et al., 2017

 

Quality Assessment of Blood Collection Tubes for ccfDNA at the End of Their Shelf Life
PDF (3MB)
Ullius et al. 2019

 

Cell-free DNA Quality and Quantity Assessment – A Method Comparison
PDF (3MB)
Ullius et al. 2019

 

Safety Data Sheets (1)
Safety Data Sheets
Download Safety Data Sheets for QIAGEN product components.
Kit Handbooks (1)
PAXgene Blood ccfDNA Tube (RUO) Handbook
PDF (328KB)
PAXgene Blood ccfDNA Tube (RUO) Handbook
Application Notes (2)
PAXgene Blood ccfDNA System Transport Concept
PDF (1MB)

IATA-conform transport of whole blood samples in PAXgene Blood ccfDNA Tubes – a simulation and functional test

Multimodal analysis of CTC RNA, ccfDNA and gDNA from a single blood sample collected into a PAXgene Blood ccfDNA Tube (RUO)
PDF (1MB)

Demonstrated 3-in-1 workflow for the simultaneous extraction of circulating tumor cell RNA, ccfDNA and leukocyte-derived gDNA from a single blood sample collected and stored in a PAXgene Blood ccfDNA Tube (RUO)

Supplementary Protocols (1)
Purification of genomic DNA from the PAXgene® Blood ccfDNA Tube using the QIAamp® DNA Blood Mini Kit - (EN)
PDF (509KB)

FAQ

Which protocol line should I use to process plasma samples?
The QIAsymphony PAXgene Blood ccfDNA Kit can be sued with 2 protocol lines. The standard protocols (STA) predominantely isolate small ccfDNA fragments. The large fragment protocols (LAF) efficiently co-purify large and small ccfDNA fragments. Both lines include a protocol for 2.4 ml and another for 4.8 ml plasma input.
FAQ ID - 3654
Which elution volumes are available with the QIAsymphony PAXgene Blood ccfDNA Kit?
The elution volume of the standard protocols (STA) is fixed at 60 µl. The large fragment protocols (LAF) enable elution in 3 different volumes: 60, 100 and 150 µl.
FAQ ID - 3655
What is the shelf life of the QIAsymphony PAXgene Blood ccfDNA Kit?
The expiration date is printed on the kit label and encoded in the bar code of the reagent cartridges.
FAQ ID - 3651
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures above 25°C?
Whole blood drawn into PAXgene Blood ccfDNA Tubes can be stored for at least 24 hours at 35°C. Higher temperatures will lead to lysis of blood cells and release of genomic DNA into plasma.
FAQ ID - 3640
Which downstream applications have been tested with ccfDNA purified from whole blood collected and processed with the PAXgene Blood ccfDNA System?
To date, ccfDNA from blood samples collected and processed using the PAXgene Blood ccfDNA System have been successfully tested in PCR, including multiplex qPCR, and NGS applications.
FAQ ID - 3658
Which tubes are recommended for the QIAsymphony sample rack
The recommended sample input format is the 14 ml Falcon® polystyrene round bottom tube 17 x 100 mm supplied by Corning (formerly supplied by BD).
FAQ ID - 3653
Can whole blood be frozen in PAXgene Blood ccfDNA Tubes?
No. Freezing blood drawn into PAXgene Blood ccfDNA Tubes results in lysis of red and white blood cells and release of genomic DNA.
FAQ ID - 3642
Are PAXgene Blood ccfDNA Tubes sterile?
Yes, the inside of PAXgene Blood ccfDNA Tubes is sterilized during production.
FAQ ID - 3636
What is the shelf life of unused PAXgene Blood ccfDNA Tubes?
The expiration date is printed on the tube label and on the shelf carton. 
FAQ ID - 3633
How long after first use can a reagent cartridge be stored for further use?
When closed properly with the provided strips and stored at room temperature (15–25°C), a reagent cartridge can be used up to 6 weeks after the initial run.
FAQ ID - 3650
Can whole blood in PAXgene Blood ccfDNA Tubes be stored at temperatures below 15°C?
Yes. Whole blood in PAXgene Blood ccfDNA Tubes can be stored at 8–15°C for up to 7 days. Storing blood in PAXgene Blood ccfDNA Tubes below room temperature does not extend stability. 
FAQ ID - 3641
What should I do if cryoprecipitates form while thawing a frozen plasma sample?

If cryoprecipitates cannot be resolved completely by vortexing, the sample should be centrifuged to prevent precipitates from blocking the column. To ensure that no cfDNA is co-precipitated,  centrifugation can be performed at a lower speed (at 6,800xg for 3 minutes at room temperature, as stated in the Protocol Sheet for the QS DSP Circulating DNA Kit). 



FAQ ID - 3646
How long and at which temperature can plasma generated from whole blood in PAXgene Blood ccfDNA Tubes be stored?
Plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes can be stored at 2–8°C for up to 14 days. We recommend freezing plasma at −20°C or −80°C for longer storage periods (long-term storage studies are ongoing).
FAQ ID - 3644
What is the expected ccfDNA yield from plasma generated from 10 ml of blood?
The yield of ccfDNA depends on the target ccfDNA. Nevertheless, yields obtained from PAXgene Blood ccfDNA Tubes are comparable to those obtained from the same volume of plasma separated directly after drawing blood into a spray-dried EDTA tube. In the PAXgene Blood ccfDNA Tube, however, this yield remains stable for up to 7 days when the tube is stored at 15–25°C or for up to 24 hours when stored at 35°C. In contrast, genomic DNA is released from lysed and apoptotic cells in the unstabilized contents of the EDTA tube soon after blood draw.


FAQ ID - 3637
Do I need to use a blood collection set with the PAXgene Blood ccfDNA Tube?
Yes. Further information on phlebotomy technique with the PAXgene Blood ccfDNA Tube can be found in the PAXgene Blood ccfDNA Tube Product Circular under the Resources tab.
FAQ ID - 3626
How many extractions can I perform with a QIAsymphony PAXgene Blood ccfDNA Kit, using the 2.4 ml or the 4.8 ml protocols?
The number of extractions that can be performed with a kit is independent of sample volume. A kit contains sufficient reagents for 192 extractions.
FAQ ID - 3649
Can reagent from the PAXgene Blood ccfDNA Tube flow back into the patient’s arm?
If the instructions for use are followed, there is no possibility of backflow during phlebotomy and thus, no possibility of reagent going into the patient’s arm.
FAQ ID - 3629
Can I use plasma from underfilled PAXgene Blood ccfDNA Tubes?
A complete 10 ml blood draw ensures optimal results. However, depending on the downstream application and target ccfDNA fragment size, tubes filled with as little as 6.5 ml whole blood can be used without adversely affecting results.
FAQ ID - 3638
Can I use a different brand blood collection set with the PAXgene Blood ccfDNA Tube?
Yes, as long as the phlebotomy instructions included with the PAXgene Blood ccfDNA Tubes are properly followed.
FAQ ID - 3630
What is the reagent in the PAXgene Blood ccfDNA Tube?
The PAXgene Blood ccfDNA Tube contains a proprietary formulation that prevents blood coagulation and the release of genomic DNA from blood cells into plasma. Contents of the tube may cause irritation to the eyes, respiratory system and skin. Please refer to the PAXgene Blood ccfDNA Tube Product Circular. For additional information, visit www.preanalytix.com or review the Safety Data Sheet under the Resources tab.
FAQ ID - 3632
What is the blood draw volume of the PAXgene Blood ccfDNA Tube
The blood draw volume is 10 ml and the stabilization reagent volume is 1.5 ml. Thus, a filled tube holds a volume of 11.5 ml.
FAQ ID - 3631
For what type of sample material is the QIAsymphony PAXgene Blood ccfDNA Kit intended?
The QIAsymphony PAXgene Blood ccfDNA Kit is designed to be used with plasma generated from whole blood collected into PAXgene Blood ccfDNA Tubes.
FAQ ID - 3652
How should I thaw frozen plasma samples?
Thaw the tubes at room temperature (15–25°C). To minimize the formation of cryoprecipitates, do not thaw at lower temperatures (e.g., 4°C). Alternatively, thaw plasma for 30 minutes at 30°C to completely avoid the formation of cryoprecipitates.
FAQ ID - 3645
Are there special considerations for phlebotomy when using the PAXgene Blood ccfDNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.)?

Yes. Following a standard phlebotomy protocol, blood is collected into the evacuated tube using a blood collection set and a standardized needle holder that can accommodate a 16 mm diameter tube. Make sure that blood has stopped flowing into the tube before removing the tube from the holder.

For more information on phlebotomy technique, please refer to the PAXgene Blood ccfDNA Tube Product Circular under the Resources tab.
FAQ ID - 3635
Is double centrifugation required for plasma processing?
No. However, a second centrifugation step helps remove traces of buffy coat that may be accidentally transferred when aspirating plasma after the first centrifugation step.
FAQ ID - 3643
How should eluates produced with the PAXgene Blood ccfDNA System be stored?
Eluates should be stored at −20°C (long-term storage studies are ongoing).
FAQ ID - 3657
Does the QIAsymphony PAXgene Blood ccfDNA Kit also allow eluting ccfDNA in less than 60 µl?
Yes, a customized protocol for elution with 50 µl is available on request.
FAQ ID - 3656
How long can whole blood stabilized in PAXgene Blood ccfDNA Tubes be stored at room temperature?
Whole blood drawn into PAXgene Blood ccfDNA Tubes can be stored at 15–25°C for up to 7 days.
FAQ ID - 3639
Which plasma volumes can I process with the QIAsymphony PAXgene Blood ccfDNA protocols?
The normal protocols enable processing 2.4 or 4.8 ml plasma. Other volumes can be accommodated using customized protocols. 
FAQ ID - 3648
How should unused PAXgene Blood ccfDNA Tubes be stored?
Store PAXgene Blood ccfDNA Tubes at room temperature (15–25°C) and protect them from direct sunlight.
FAQ ID - 3634
Why is a blood collection set required to collect blood into the PAXgene Blood ccfDNA Tube?
When used in accordance with instructions, the blood collection set eliminates the possibility of patient contact with the reagent in the tube due to backflow of blood.
FAQ ID - 3628

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