miRNeasy Kits enable purification of total RNA, which includes RNA from approximately 18 nucleotides (nt) upwards. Alternatively, an miRNA-enriched fraction and a total RNA (>200 nt) fraction can be purified separately (see flowchart " miRNeasy procedures"). Total RNA, including miRNA, is highly suitable for use in miRNA quantification by real-time RT-PCR. Enrichment of small RNAs in a separate fraction may be advantageous for certain applications where mRNA and rRNA could lead to increased background. This specialized protocol requires use of the RNeasy MinElute Cleanup Kit with the miRNeasy Micro or Mini Kit. If using the miRNeasy 96 Kit, 2 RNeasy 96 plates are required for every 96 samples.
Automated miRNA purification on the QIAcube Connect
Purification of total RNA, including miRNA, from the aqueous phase after phase separation can be fully automated on the QIAcube Connect using the miRNeasy Micro or Mini Kit (see figure " High, reproducible recovery of miRNA from small cell numbers"). The innovative QIAcube Connect uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into your laboratory workflow. Sample preparation using the QIAcube Connect follows the same steps as the manual procedure (i.e., lyse, bind, wash, and elute), enabling you to continue using the miRNeasy Micro or Mini Kit for purification of high-quality RNA.