A simple workflow allows the purification of ultraclean DNA from whole blood, cells, swabs or tissue (see flowchart “ QIAamp UCP DNA Micro procedure”).
Samples are lysed under highly denaturing conditions at elevated temperatures in the presence of proteinase K and Buffer AUT. DNA is then bound to the QIAamp UCP MinElute column membrane and two wash steps efficiently wash away contaminants. DNA is eluted from the QIAamp UCP MinElute column using a small volume of Buffer AUE or ultraclean PCR water. Since the elution volume is small, the eluted DNA will be concentrated.