artus VZV PCR Kits provide all necessary reagents optimized for reliable VZV DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.
Common temperature profiles for multiple assays in one run
The temperature profile of the artus VZV LC PCR, RG PCR, and QS-RGQ Kits corresponds to the profiles of the artus LC PCR, RG PCR, and QS-RGQ Kits for CMV, EBV, and HSV-1/2, as well as for BK virus (RG and QS-RGQ only). Therefore, these PCR assays can be performed and analyzed in a single run.
Complete automated system from sample to VZV detection
The QIAsymphony RGQ workflow solution for VZV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus VZV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete CE-IVD-compliant workflow (see figure " Integrated QIAsymphony RGQ system for VZV detection").
Recommendations for viral DNA purification when using the artus VZV LC, RG, or TM PCR Kits
For DNA purification, the EZ1 DSP Virus Kit is to be used or, depending on the sample type and artus VZV PCR Kit, the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit. The artus VZV RG PCR Kit is also available as CE-IVD-marked EASYartus VZV RG PCR Kits, which include the EZ1 DSP Virus Kit for automated viral nucleic acid purification.