For real-time quantitative PCR analysis of control gene, fusion gene, or mutated gene transcripts
EAC standardized assay for control and fusion gene transcripts
Optimal quality control and ISO 13485 certification
Easy workflow with ready-to-use solutions
ipsogen Standards are for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. These ready-to-use plasmid DNA standards are optimized for reliable and sensitive quantitation of control gene, fusion gene, or mutated gene transcripts.
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
ipsogen Standards precisely quantify the copy number of control gene, fusion gene, or mutated gene transcripts by generating standard curves based on the known concentration of plasmid DNA dilutions of the target gene. Each control gene standard includes 3 or 4 dilutions of a given gene.
ABL: either 3 (103, 104, 105 copies in 5 μl) or 4 dilutions (103, 104, 105, 106 copies in 5 μl)
B2M: 3 dilutions (105, 106, 107 copies in 5 μl)
BCR and GUS: 3 dilutions (103, 104, 105 copies in 5 μl)
Each fusion gene standard and mutated gene standard includes 5 dilutions of a given fusion gene or mutated gene. The dilutions provide 101, 102, 103, 105, 106 copies in 5 μl. ipsogen Standards are extremely stable DNA plasmids and are designed according to Europe Against Cancer (EAC) recommendations for control gene and fusion gene transcripts.
ipsogen Standards enable reliable and sensitive quantitation of control gene, fusion gene, or mutated gene transcripts using real-time PCR.
For processing of approximately 10 mg tagged protein: 0.5 units DAPase Enzyme, 30 units Qcyclase Enzyme, 10 units pGAPase Enzyme, 20 mM Cysteamine-HCl (1 ml), Ni-NTA Agarose (10 ml), 20 Disposable Columns
For 250 x 20 μl reactions: 3 x 0.85 ml HotStarTaq Plus Master Mix (contains 250 units of HotStarTaq Plus DNA Polymerase, PCR Buffer with 3 mM MgCl2, and 400 μM of each dNT), 1 x 0.55 ml CoralLoad Concentrate, 2 x 1.9 ml RNase-Free Water