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RNeasy Plus Micro and Mini Kits

 For phenol-free total RNA extraction from cells/tissues with removal of genomic DNA contamination
  • Efficient gDNA removal with unique gDNA Eliminator columns (no need for DNase)
  • High-quality total RNA in minutes using fast and simple extraction protocols
  • Phenol-free RNA isolation
  • Ideal for sensitive applications such as real-time RT-PCR and RNA-Seq 

The RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining high-quality RNA purification with effective on-column gDNA removal. Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. The RNeasy Plus Mini Kit purifies up to 100 µg total RNA (>200 nt) from a single extraction with efficient gDNA removal. The RNeasy Plus Micro Kit is specially designed for limited amounts of samples and isolates up to 45 µg pure total RNA. The kits can be automated on the QIAcube and samples can be conveniently stabilized using RNAprotect Tissue Reagent or Allprotect Tissue Reagent (available separately).

 

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Specifications

Features
Specifications
Applications Northern, dot, and slot blotting, end-point RT-PCR, quantitative, real-time RT-PCR, array analysis, next-generation sequencing/ RNA-Seq
Elution volume 14 µl (micro kit), 30-50 µl (mini kit)
Format Spin column
Integrated removal of genomic DNA Yes
Main sample type Tissue, cells
Processing Manual (centrifugation) – automatable on QIAcube
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA
Sample amount 5 x 10e5 cells or 5 mg tissue (micro kit), 10e7 cells or 30 mg (<700 µl) (mini kit)
Technology Silica technology
Time per run or per prep 25 min
Yield Varies

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Supplementary Protocols (2)

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There are two protocols: follow Protocol 1 if you want to purify total RNA containing miRNA, or follow Protocol 2 if you want to purify small RNA (includes miRNA, 5S rRNA, and tRNA) and larger RNA (>200 nt) separately.
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Scientific Posters (1)
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