What are the expected DNA yields from different tissues using the QIAamp DNA Mini Kit?
FAQ ID -45
The expected DNA yields from different tissues using the QIAamp DNA Mini Kit are as follows:
| Sample |
Nucleic acid yield without RNase A treatment (ug) |
DNA yield with RNase A treatment (ug) |
| Blood (200 ul) |
4–12 |
4–12 |
| Buffy coat (200 ul) |
25–50 |
25–50 |
|
Cultured cells (5 x 10e6) |
20–30 |
15–20 |
| Liver (25 mg) |
60–115 |
10–30 |
| Brain (25 mg) |
35–60 |
15–30 |
| Lung (25 mg) |
8–20 |
5–10 |
| Heart (25 mg) |
25–45 |
5–10 |
| Kidney (25 mg) |
40–85 |
15–30 |
| Spleen (10 mg) |
25–45 |
5–30 |
DNA was purified with QIAamp Kits following standard protocols.