What is the genotype of the EZ Competent Cells?
FAQ ID -157
Genotype of QIAGEN EZ Competent Cells supplied in the QIAGEN PCR Cloning plus Kit:
| Genotype |
Description/phenotype |
| F' |
Presence of the low-copy-number F plasmid [F'::Tn10(Tcr) proA+ B+ lacIq Z delta-M15] |
| Tn10 (Tcr) |
Transposon conferring tetracyclin resistance |
| lacIq |
Overproduction of the lac operon repressor, which controls expression from Plac |
| lacZ delta-M15 |
Expression of N-terminally deleted beta-galactosidase.This protein and LacZ alpha-peptide (encoded by the pDrive Cloning Vector) provide alpha-galactosidase activity. Cells transformed by pDrive Cloning Vector which does not contain a PCR product will express LacZ alpha-peptide, and will form blue colonies when grown in the presence of X-gal/IPTG. In contrast, cells transformed by pDrive Cloning Vector which does contain a PCR product will not express LacZ alpha-peptide, and will form white colonies. |
| recA1 |
Abolished homologous recombination |
| end A1 |
Abolished nonspecific endonuclease I activity, and hence improved DNA quality in plasmid preparations |
| hsdR17 |
This mutation prevents restriction, but not protective methylation, of unmethylated DNA or DNA containing foreign methylation patterns in E. coli cells. |
| lac |
Inability to utilize lactose |
| glnV44 |
Supressor of amber (UAG)mutation; required for growth of some phage vectors. Formerly called supE |
| thi-1 |
Requires thiamin (vitamin B1) for growth in minimal medium |
| gyrA96 |
Mutation in DNA gyrase; resistance to naladixic acid |
| relA1 |
Relaxed mutation, permits RNA synthesis in the absence of protein synthesis |
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