The miRCURY LNA miRNA PCR System uses one single cDNA synthesis reaction for all amplifications, reducing pipetting and saving time and sample. Two LNA-enhanced miRNA-specific qPCR primers enable highly specific and sensitive amplification and single nucleotide discrimination. The fast and easy workflow takes only 3 hours with minimal hands-on steps.
One universal RT reaction per RNA sample saves not only reagents and precious samples, but also your time.
Data from serial dilutions of human AM6000 total RNA are shown. All miRNA assays exhibit linear readout with correlation coefficients R2 > 0.99.