AllStars Hs Cell Death Control siRNA

For siRNA transfection optimization and positive control experiments in human cells

Features

  • Optimization and positive control experiments made easy
  • Valuable control for high-throughput RNAi
  • Simple evaluation by light microscopy
  • Highly effective for a wide range of cell types

Products

The AllStars Hs Cell Death Control siRNA is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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AllStars Hs Cell Death siRNA (1 nmol)

Cat. No. / ID: SI04381048

Positive cell death phenotype control, 1 nmol
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AllStars Hs Cell Death siRNA (5 nmol)

Cat. No. / ID: 1027298

Positive cell death phenotype control, 5 nmol
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AllStars Hs Cell Death siRNA (20 nmol)

Cat. No. / ID: 1027299

Positive cell death phenotype control, 20 nmol

Product Details

AllStars Hs Cell Death Control siRNA is a blend of highly potent siRNAs targeting ubiquitously expressed human genes that are essential for cell survival. Knockdown of these genes induces a high degree of cell death, which is visible by light microscopy. Transfection efficiency can be quickly estimated by simply observing cells by straightforward light microscopy 48–96 hours after transfection of AllStars Hs Cell Death Control siRNA, avoiding the need for any complex or laborious downstream assay. AllStars Hs Cell Death Control siRNA is a useful tool for siRNA transfection optimization and can be used routinely as a positive control.

Performance

Fast and easy detection in a wide range of cell types

The performance of AllStars Hs Cell Death Control siRNA is validated for a wide range of cell types (see table).

AllStars Hs Cell Death Control siRNA Validated Cell Types
Cell line Cell type
293 Human embryonic kidney cells
A549 Human alveolar basal epithelial cells
CaCo Human colonic carcinoma cells
HeLa Human cervical cancer cells
HeLa S3 Human cervical cancer cells
HepG2 Human hepatoma cells
Huh7 Human hepatoma cells
MCF-7 Human breast adenocarcinoma cells
MDA-MB-231 Human breast adenocarcinoma cells
ME180 Human cervical squamous carcinoma cells
SW 480 Human colon adenocarcinoma cells
Primary cells Cell type
HUVEC Primary human umbilical vein endothelial cells
NHBE Primary normal human bronchial epithelial cells
NHLF Primary normal human lung fibroblast cells
Highly efficient knockdown and cell death

Knockdown efficiency using AllStars Hs Cell Death Control siRNA has been quantified by real-time RT-PCR and is >95% for each target mRNA.

The AllStars Hs Cell Death Control significantly outperforms other commercially available toxic control siRNAs in both the level of cell death induced and in the wide range of cell types in which it has been validated. Consistently high levels of cell death are achieved using AllStars Hs Cell Death Control, ensuring effective control experiments (see figure  High level of cell death).

See figures

Principle

Easy transfection optimization

When establishing RNAi in start-up experiments or in a new cell line, multiple transfections should be performed under different conditions to determine the optimal conditions for maximum transfection efficiency. These experiments can be performed using AllStars Hs Cell Death Control siRNA (see figures  Easy siRNA transfection optimization and  Cell death in primary cells). Transfection conditions that result in the greatest degree of cell death compared with transfection with a nonsilencing control siRNA can be maintained in future experiments.

Reliable high-throughput RNAi

In high-throughput RNAi screens, the use of internal positive controls is essential to ensure that optimal transfection conditions are maintained on every plate of the screen. Positive controls that can be analyzed phenotypically have the advantage of allowing transfection efficiency to be observed immediately. Using AllStars Hs Cell Death Control siRNA on every plate allows rapid and inexpensive estimation of the transfection rate on individual plates.

See figures

Procedure

Using AllStars Hs Cell Death Control siRNA, transfection efficiency can be quickly estimated by simply observing cells by straightforward light microscopy 48–96 hours after transfection, avoiding the need for any complex or laborious downstream assay.

AllStars Hs Cell Death Control siRNA can be ordered in tube format in 1 nmol, 5 nmol, or 20 nmol amounts. This control is also available in 96-well plates and 384-well plates (FlexiPlate siRNA) in 1 nmol, 0.25 nmol, or 0.1 nmol amounts.

Applications

  • Start-up RNAi experiments
  • Transfection optimization
  • High-throughput RNAi

Supporting data and figures

Specifications

FeaturesSpecifications
DesignDesigned using HP OnGuard siRNA design
FormatTube
Scale or yield5 nmol, 20 nmol
Target sequence providedNo
ModificationYes
SpeciesHuman

FAQ

Which controls can I choose for FlexiPlate siRNA?

You can add the following controls to your FlexiPlate siRNA plate: AllStars Negative Control siRNA, AllStars Cell Death Control siRNA, Negative Control siRNA, Human GAPDH siRNA, Human Beta-Actin siRNA, Human and mouse MAPK1 siRNA, Human or mouse Lamin A/C siRNA, Mouse AKT1 siRNA, or other siRNAs from GeneGlobe, such as HP Validated siRNAs.

FAQ ID -1368
Which siRNA controls are available in FlexiTube format?
siRNA Controls available in FlexiTube format are:

Mm/Hs_MAPK1 control siRNA (5 nmol)    cat. no. 1022564

GFP-22 siRNA (5 nmol)    1022064

Luciferase GL2 siRNA (5 nmol)     1022070

Luciferase GL3 siRNA (5 nmol)     1022073

Negative Control siRNA (5 nmol)      1022076

AllStars Negative Control siRNA (5 nmol)     1027280

AllStars Neg. Control siRNA (20 nmol)        1027281

AllStars Neg. siRNA AF 488 (5 nmol)         1027284

AllStars Neg. siRNA AF 546 (5 nmol)         1027285

AllStars Neg. siRNA AF 555 (5 nmol)         1027286

AllStars Neg. siRNA AF 647 (5 nmol)          1027287

AllStars Neg. siRNA AF 488 (20 nmol)         1027292

AllStars Neg. siRNA AF 546 (20 nmol)          1027293

AllStars Neg. siRNA AF 555 (20 nmol)          1027294
 
AllStars Neg. siRNA AF 647 (20 nmol)          1027295

AllStars Hs Cell Death Control siRNA (5 nmol)     1027298

AllStars Hs Cell Death Control siRNA  (20 nmol)    1027299

Negative Control siRNA (20 nmol)                 1027310

Mm/Hs_MAPK1 control siRNA (20 nmol)          1027321







FAQ ID -1661
Do you have FACS or Fluoro-Microscopy data using HiPerFect HTS Reagent for delivery of siRNA?
No, we have qRT-PCR and phenotypic data (using AllStars Cell Death Control siRNA). We do not have data with fluorescently labeled siRNA.
FAQ ID -2557