| Cell Line Species/Tissue: |
Neuroblastoma
/
Neuroblastoma |
| Transfection Reagent: |
HiPerFect |
Nucleic Acid:
|
siRNA (dsRNA) |
Growth Medium:
|
RPMI + 10%FCS + 1% P/S |
Percent Serum (%):
|
10 % |
Reporter System:
|
|
Plasmid Purification Method:
|
|
Plate Format:
|
24-well plate |
Number of Cells:
|
40.000 |
Percent Confluence(%):
|
50% |
Amount of Nucleic Acid (µg):
|
0,9 nM |
Amount of Enhancer (µl):
|
|
Amount of Reagent (µl):
|
3 µl |
Complex Incubation on Cells (hrs):
|
48 h |
Analysis Performed Post-Transfection (hrs):
|
|
Transfection Efficiency (%):
|
|
| Knockdown Efficiency (%): |
|
| |
| Any modifications to the protocol?:
|
| no modifications |
| Notes: |
| Knockdown analysis: qPCR or qRT-PCR/
Target: VEGF-A |
| References: |
| |