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RT2 qPCR Primer Assay Service

For comprehensive gene expression analysis
  • Primer assays are available for multiple species
  • Project completion and success ensured
  • Guaranteed fast delivery
  • Outstanding data quality
  • Each project is thoroughly reviewed and quality controlled
The RT2 qPCR Primer Assay Service offers comprehensive gene expression services based on RT2 qPCR Primer Assays that are available for every human, mouse, rat, drosophila, and Rhesus macaque gene. Each RT2 qPCR Primer Assay has been experimentally verified to amplify a single amplicon of the correct size with uniform PCR efficiency. In addition, the RT2 qPCR Primer Assay Service offers custom design and verification services enabling analysis of any gene regardless of species. Verified procedures and optimized processes yield high-quality data in a rapid turnaround time.
Cat No./ID: RT2qPCR
RT² qPCR Primer Assay Service
The RT2 qPCR Primer Assay Service is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Principle

The RT² qPCR Primer Assay is a SYBR® Green-based quantitative real-time PCR assay for gene expression analysis. Our experimentally verified design algorithm yields gene-specific qPCR assays characterized by uniform and high PCR efficiencies and standardized amplification conditions. Every RT² qPCR Primer Assay is subjected to rigorous experimental verification. Single product amplification of the correct size and high PCR efficiency are guaranteed. The uniform PCR efficiencies and PCR conditions of the RT² qPCR Primer Assays provide an accurate and scalable solution for multiple gene expression analyses.

Procedure

Recommended sample types and sample collection details are described below.

For all samples, sample collection should be carried out in a timely manner in an RNase-free environment. Please note the minimum amount of starting materials required (see below for requirements for each sample type). If you have less than the minimum amount or your previously isolated samples have been stored differently than we recommend, please contact QIAGEN Technical Services before submitting your samples.

Before shipping: 

Obtain a sample submission form from QIAGEN Technical Services.
Complete the form and include it with the sample shipment.
Include the amount of material and its storage conditions. 
Sign and date the form. 
Ship according to the instructions for your sample type.
Total RNA

Where total RNA samples are submitted, a minimum of 1 µg RNA per sample is preferred, at a minimum concentration of 50 ng/µl. If samples contain less RNA or the RNA concentration is below 50 ng/µl, contact QIAGEN Technical Services.

We recommend a Bioanalyzer RIN of no less than 7, if a quality analysis was previously performed. QIAGEN will measure RNA concentration and assess the quality of the submitted RNA using an Agilent 2100 Bioanalyzer with an RNA 6000 NanoChip.

If the submitted samples are cells or tissue, an RNA Isolation Service is available. 

Cells

Provide at least 0.5 million (106) cells for each sample (specify number of cells on the submission form). Snap freeze the cell pellets in liquid nitrogen, store samples frozen at –80°C, and ship on dry ice.

Tissue

Provide at least 50 mg of tissue for each sample (specify the weight and type of tissue on the submission form). Snap freeze tissues in liquid nitrogen. Store samples frozen at –80°C, and ship on dry ice.

Other sample types

Please contact QIAGEN Technical Services.

Sample processing

RNA samples are processed by QIAGEN for RT2 qPCR Primer Assays using the following steps:

RNA isolation and QC (optional)
Reverse-transcription using the RT² First Strand Kit (FFPE samples, LCM, FNAB, and other small samples may require preamplification using RT² PreAMP cDNA Synthesis Kit and RT² PreAMP Pathway Primer Mixes) 
Individual qRT-PCR assay service: 100% verified, available for all human, mouse, rat, drosophila, and Rhesus macaque genes. Service with a custom design and validation service available for other species, genes, or other types of projects (viral, bacterial, etc.) is also available. 
Data analysis using dedicated PCR array data analysis software 
Data report delivered via email or secure FTP site
Data analysis

Data analysis is performed using dedicated qRT-PCR data analysis software. Data in an electronic form can be downloaded from a secure FTP Website or delivered via email. A complete data and experiment report includes: 

Raw CT values 
ΔΔCT analysis 
Excel spreadsheet

Contact a Technical Support representative to order or to discuss your specific need at support@sabiosciences.com.


Applications
The RT2 qPCR Primer Assay Service is highly suited for applications requiring quantitative gene expression analysis.

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