QIAGEN Sequencing Services
For the highest standards in contract DNA sequencing
QIAGEN offers a wide range of sequencing services. Our single-read services enable routine sequencing of plasmids, PCR products, and expressed sequence tags (ESTs). QIAGEN's verification services allow resequencing and comparison with reference sequences, while our de novo sequencing services enable analysis of unknown sequences, from single stranded primer walking to patent quality. Our genome services are suitable for custom/tailored projects abd allow access to genomic sequence information. Additonal special services include siRNA hairpin runs, genomic DNA sequencing, mutation detection, DNA purification, bioinformatics, and whole genome amplification.
Performance
QIAGEN delivers the highest standard in contract DNA sequencing.
QIAGEN Sequencing Services — Single-Read Services Single-read sequencing services (96-well format) are ideal for small-scale analysis of cDNAs and ESTs, identification of clones, and verification of vector/insert transitions. All single read services include extensive documentation of the sequencing data, which is available via e-mail, FTP file, or on CD. Easy-read sequencing Easy-read sequencing is the service of choice for standard sequencing reactions. This highly automated service allows rapid processing of purified DNA samples. Easy-read sequencing is offered as tube service, or as a plate service for sequencing of samples in batches of 96. Easy-read sequencing does not include template purification (i.e., purification of PCR fragments or plasmid preparations prior to sequencing reaction setup). Sequencing reactions are set up using the DNA concentration information provided by the customer. All sequencing results are subjected to quality control using Phred20 algorithms (typically 600–900 bases of Phred20 controlled sequence are obtained). Quality documentation including raw data and quality clipped sequence data is delivered via e-mail, ftp, or on CD. Failed sequences are not repeated. This service provides high-quality single-stranded sequencing data 1–2 working days after receipt of purified DNA. Easy-sead sequencing provides: • Single sequencing reaction • Data collection to maximum read length • Typical read length up to 600–900 nt • Phred20 quality • >99% accuracy • Without template purification • Without incoming DNA quality check • No repeat reaction • Documentation of sequencing results Single-read sequencing If your sequencing reaction needs special attention we recommend our single-read sequencing Service. The sequencing results are manually checked and any failed sequencing reactions are repeated using modified sequencing parameters. This means that you get the most reliable service even from difficult samples (e.g., samples with high GC content). This service is offered with or without template purification as a plate service for sequencing of samples in batches of 96. Single-read sequencing with template preparation includes the purification of PCR fragments or plasmids prior the sequencing reaction setup. Templates are quality controlled to ensure an optimal sequencing reaction setup. All sequencing results are subjected to quality control using Phred20 algorithms (typically 600–900 bases of Phred20 controlled sequence are obtained). Quality documentation including raw data and quality clipped sequence data is delivered via e-mail, ftp, or on CD. Failed sequences are inspected by our experienced team and repeated using modified sequencing parameters. This service provides high-quality single-stranded sequencing data in 2–3 working days (3–4 working days with template purification). Single-read sequencing provides: • Single sequencing reaction • Incoming DNA quality check • Repeat reaction, if necessary • Data collection to maximum read length • Read length up to 600–900 nt • Phred20 quality • >99% accuracy • Documentation of sequencing results BAC end-sequencing End-sequencing of BACs, PACs, or cosmids. The results are manually checked by our experienced team and failed sequencing reactions are repeated using modified sequencing parameters. This means that you get the most reliable service even from difficult samples (e.g., samples with high GC content). This service is offered with or without template purification as a plate service for sequencing of samples in batches of 96. The delivery time is approximately 2–3 working days (3–4 working days with template purification). BAC End-Sequencing provides: • Single sequencing reaction • Incoming DNA quality check • Repeat reaction if necessary • Data collection to maximum read length • Read length up to 600–900 nt • Phred20 quality • >99% accuracy • Documentation of sequencing results siRNA hairpin run siRNA expression vector templates exhibit secondary structures that are often difficult to sequence. QIAGEN has developed a special sequencing reaction that enables accurate sequencing of siRNA expression vector templates. The results are manually checked by our experienced team and failed sequencing reactions are repeated using modified sequencing parameters. This service is offered with or without template purification as a plate service for sequencing of samples in batches of 96. The delivery time is approximately 2–3 working days (3–4 working days with template purification). siRNA hairpin run provides: • Single sequencing reaction • Incoming DNA quality check • Repeat reaction if necessary • Data collection to maximum read length • Read length up to 400-600 nt • Phred20 quality • >99% accuracy • Documentation of sequencing results
QIAGEN Sequencing Services — Verification Services Verification sequencing Verification sequencing provides confirmation of an already known DNA sequence, as well as detection of mutations or deletions. Both DNA strands are completely sequenced using custom primers based on a provided DNA sequence. The sequence is verified against the provided reference sequence. Verification sequencing provides:
Verification sequencing Under GLP-like conditions Verification sequencing Under GLP-like conditions provides confirmation of an already-known DNA sequence including comprehensive documentation of the sequencing results. Both DNA strands are completely sequenced to at least 4-fold coverage. The sequence is verified against a provided reference sequence. Documentation can be used for patent applications, litigations, or FDA submissions. GLP-like verification sequencing comprises:
QIAGEN Sequencing Services — De Novo Sequencing QIAGEN offers a number of de novo Sequencing services, providing accurate sequence data from unknown DNA. Sequencing quality ranges from single stranded primer walking for preliminary analysis to complete sequence analysis for patent submission. De novo publication-ready sequencing The de novo publication-ready sequencing service provides publication-ready DNA sequence from unknown template DNA. Both DNA strands are completely sequenced and the sequence is fully edited and assembled. Depending on the project size, different sequencing strategies can be applied (i.e., primer walking for plasmids or shotgun sequencing cosmids, BACs, or PACs). If you provide us with an E. coli host strain harbouring the construct of interest, we will apply the best strategy to determine the sequence and we will send back the sequencing results within 20 working days. De novo publication-ready sequencing provides:
De novo investigation-grade sequencing De novo investigation-grade sequencing provides a single stranded primer walking approach for preliminary analysis of unknown sequences. The target DNA is sequenced to single-strand coverage and the sequence is edited and assembled. We guarantee a sequence quality score of Phred20 (i.e., an error rate of 1:100 nucleotides) for all assigned bases. De novo investigation-grade sequencing provides:
De novo publication sequencing under GLP-like conditions De novo publication sequencing under GLP-like conditions provides double stranded sequencing including comprehensive documentation. Both DNA strands are completely sequenced to at least four fold coverage. Documentation can be used for patent applications, litigations, or FDA submissions. If you provide us with an E. coli host strain harbouring the construct of interest, we will apply the best strategy to determine the sequence and we will send back the sequencing results within 20 working days. De novo publication sequencing Under GLP-like conditions provides:
QIAGEN Sequencing Services — Genome Services QIAGEN’s genome service provides large-scale genomic DNA sequencing including the sequencing of whole genomes. The genome aervice provides:
QIAGEN sequencing services has become a well-established participant in many publicly funded and private genome projects. The following table shows our contribution to selected projects:
One critical factor for successful genome sequencing is the generation of whole genome shotgun libraries. Our shotgun libraries are of an extremely high quality. Shotgun libraries with different insert sizes are used to establish the sequence either up to publication quality (>99.995% sequence accuracy) or up to a certain genomic coverage. We also offer various bioinformatic analyses including complete annotation and comparative sequencing. For large-scale projects, a dedicated project management plan and team is instigated to carry out all necessary work in due speed and to the highest accuracy and quality levels. QIAGEN Sequencing Services — Special Services QIAGEN offers a number of special services that complement our sequencing service. These services include the sequencing of templates with a high degree of secondary structure, the sequencing of genomic DNA, the detection of mutations, DNA purification, whole genome amplification, and bioinformatic analysis. siRNA hairpin run siRNA expression vector templates exhibit secondary structures that are often difficult to sequence. QIAGEN has developed a special sequencing reaction that enables accurate sequencing of siRNA expression vector templates. The results are manually checked by our experienced team and any failed sequencing reactions are repeated using modified sequencing parameters. This service is offered with or without template purification as a plate service for sequencing of samples in batches of 96. The delivery time is approximately 2–3 working days (3–4 working days with template purification). siRNA hairpin run provides:
Genomic DNA Sequencing Primer walking directly on genomic DNA provides a means of efficiently closing gaps between contigs. We use special reaction parameters to directly sequence prokaryotic or viral DNA with genome sizes <5 mb. Genomic DNA sequencing provides:
Mutation detection service Sequencing remains the method of choice for mutation detection and profiling. QIAGEN Sequencing Services offer a Mutation detection service tailored to your specific needs. Our bioinformatics experts will perform all steps including assay design and primer synthesis, PCR optimization and amplification, DNA purification, bi-directional DNA sequencing, quality scoring, automated mutation calling, and sequence analysis. The mutation detection service provides:
DNA purification service Don't waste your time with routine work. QIAGEN offers both small- and large-scale DNA preparation services from a variety of different starting materials. The DNA purification service provides:
Whole genome amplification service If you do not have enough starting material for your downstream analysis, we recommend amplification of your genomic DNA. The REPLI-g service — based on proven REPLI-g whole genome amplification technology — allows the amplification of unlimited amounts of DNA from limited samples with minimal sequence bias. A stringent quality control assay provides information on the quality of the amplified DNA, enabling reliable predictions for the success of your downstream assay to be made. For more information, or to find out how to take advantage of this service click here. Bioinformatic analysis Every sequencing project has special requirements for bioinformatic resources (e.g., large scale BLAST analysis, EST clustering, custom primer design, or annotation). The QIAGEN Sequencing Service provides in-depth bioinformatic analysis of DNA sequences for sequencing projects of any scale
Principle
Simply send your sample and take advantage of QIAGEN's expertise. From sample receipt, DNA purification and QC of purified DNA to sequencing and data analysis and delivery, QIAGEN offers a one-stop service for all your contract manufacturing and sequencing needs.
Procedure
To get started, obtain a quotation for your project request. If you are a new customer, please contact QIAGEN Sequencing Services (QSS) for pricing information.
Complete an order request Please download our order form in Excel format. Order forms in excel format should be e-mailed to sequencing@qiagen.com in advance. A hardcopy MUST BE submitted with every package shipped to QIAGEN. Sample submission and shipping Please provide the following DNA concentrations per sequencing reaction:
Procedures for shipping clones and DNA from countries outside the EU to QIAGEN Sequencing Services QIAGEN has selected FedEx as our preferred carrier to Germany. Please follow this checklist carefully to ensure that your samples are processed efficiently through FedEx and German customs. A padded shipping envelope may be used for small numbers of tubes or plates. For larger shipments, boxes may be used but must accommodate the FedEx International Waybill and Commercial Invoice.
Andre Bahr: +49-2103-29-16345 Anke Henne: +49-2103-29-16234 Retrieval of results DNA sequencing results are made available via e-mail or secure (password protected) web download, typically 24-48 hours after samples have arrived in our labs. Contact/shipping information Please send your samples to: QIAGEN Genomic Services Sequencing Services QIAGEN Str. 1 D-40724 Hilden Germany IMPORTANT: Do not forget to indicate the sender when you send your samples! This information is essential so that we can correctly match your samples to your order form. Alternatively, please include a hard copy of the order form when you send your samples to us. You will receive an acknowledgement of your order by e-mail when we receive your samples. If you have any questions, please do not hesitate to call your local Technical Service or the Genomic Services Department in Germany at +49 2103 29 16234 or +49 2103 29 16345.
Applications
QIAGEN Sequencing Services can be used for:
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