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MagAttract Viral RNA M48 Kit

For automated purification of viral RNA from up to 300 µl serum or plasma samples

  • Rapid purification of high-quality viral RNA
  • Flexible purification of 6–48 samples per run
  • Easy optimization of RNA concentration

The MagAttract Viral RNA M48 Kit provides automated purification of viral RNA on the BioRobot M48 using proven MagAttract magnetic particle technology. Variable elution volumes enable easy optimization of viral RNA concentration for downstream applications. Viral RNA purification and magnetic separation take place in the pipet tips, increasing the efficiency of the procedure.

Cat No./ID: 955235
MagAttract Viral RNA M48 Kit (96)
€416.00
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For 96 viral RNA preps: MagAttract Suspension F, buffers
The MagAttract Viral RNA M48 Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
Performance

The yield of viral RNA per sample with the MagAttract Viral RNA M48 Kit is normally below 1 µg, and is therefore difficult to determine with a spectrophotometer. Quantitative amplification methods are recommended for determination of yields.

Principle

The MagAttract Viral RNA M48 Kit provides fully automated purification of viral RNA from serum and plasma. MagAttract technology combines the speed and efficiency of silica-based RNA purification with the convenient handling of magnetic particles. Samples are processed in the BioRobot M48 in 1.5 ml or 2 ml sample tubes. Up to 48 samples in increments of 6 samples can be processed per run. The sample source can be up to 300 µl serum or plasma. The yield of purified RNA depends on the sample type and virus titer.

Procedure

In the MagAttract Viral RNA M48 procedure, viral RNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. Viral RNA bound to the particles is then efficiently washed using two different wash buffers. High-quality viral RNA is eluted at 65ºC in an RNase-free elution buffer.

Applications

The high-quality RNA obtained using the MagAttract Viral RNA M48 Kit and the BioRobot M48 is suited for use in many downstream applications, such as:

Viral epidemiology and genotyping
Infectious disease research
Features
Specifications
Applications PCR, real-time, genotyping, sequencing
Elution volume 50–100 µl
For automated processing BioRobot M48 Workstation
Format Filter tip (up to 48 per run)
Main sample type Serum, plasma
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Viral RNA
Sample amount 300 µl
Technology Magnetic particles
Yield Varies
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