text.skipToContent text.skipToNavigation

Qproteome Mitochondria Isolation Kit

For efficient isolation of high-purity mitochondria preparations
  • High-purity, intact mitochondria
  • Highly reproducible using a standardized procedure
  • Convenient, fast, and easy-to-use kit format

The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Cat No./ID: 37612
Qproteome Mitochondria Isolation Kit
€339.00
Add To Cart
Buffers and reagents for 12 high-purity mitochondrial preparations
The Qproteome Mitochondria Isolation Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
0
More intact mitochondria per milligram protein.

Mitochondria were isolated using the Qproteome Mitochondria Isolation Kit or another commercially available kit (Supplier S) according to manufacturer’s instructions. Mitochondrial membrane integrity is indicated by formation of fluorescent aggregates following uptake of a carbocyanine dye.

1
High purity mitochondria with intact mitochondrial membranes.
[A] Mitochondria were prepared from 60 mg of liver tissue using the Qproteome Mitochondria Isolation Kit and subjected to 2D-PAGE. Out of 384 spots picked, 259 proteins were identified by MALDI-MS, 219 (85%) of which were predicted to have a mitochondrial localization. Green circles indicate mitochondrial proteins, red circles indicate impurities, the black circle indicates a protein which is probably located in mitochondria and ER. [B] Electron micrographs of mitochondria isolated using the Qproteome Mitochondria Isolation Kit showing intact inner and outer membranes.
2
Mitochondrial fractions virtually free of cross-contamination.
Western blots of mitochondrial preparations probed with antibodies specific for organelle-/cell-compartment specific marker proteins. PM: plasma membrane; ER: endoplasmic reticulum.
Performance
In contrast to a differential centrifugation method from the literature, analysis of cell-compartment marker proteins shows that the Qproteome Mitochondria Isolation Kit provides mitochondrial fractions virtually free of cross-contamination (see figure "Mitochondrial fractions virtually free of cross-contamination"). The virtual absence of cytosolic contaminants greatly facilitates analysis of low-abundance species as shown in 2D-PAGE analyses and the gentle procedure provides a highly enriched fraction containing intact mitochondria (see figure "High purity mitochondria with intact mitochondrial membranes"). Integrity can also be demonstrated by measuring uptake of a fluorescent dye (see figure "More intact mitochondria per milligram protein").
Principle

One of the major problems facing researchers working with mitochondria is efficiently separating mitochondrial and cytosolic fractions. Traditional preparation methods are tedious, time-consuming, and require access to an ultracentrifuge. The Qproteome Mitochondria Isolation Kit provides fully active mitochondrial preparations of very high purity in a fast, reproducible, and standardized procedure that uses a standard bench-top centrifuge.

Applications

Mitochondria are the subject of intense study in a number of fields, including apoptosis, proteomics, biomedical research, and drug discovery. The Qproteome Mitochondria Isolation Kit delivers high-purity mitochondria whose proteins retain their native conformation and full biological activity — making them highly suited for apoptosis studies, 2D-PAGE analysis, and other biochemical assays.

Features
Specifications
Applications SDS-PAGE, mass spectrometry, activity assays
Binding capacity/yield 20–80 µg
For glycoproteins: which type of glycoproteins n.d
Fractions isolated One fraction
Sample size 5 x 10e6 - 2 x 10e7 cells, 20 mg - 60 mg
Species Eukaryotes
Start material Cells, Tissue

You are not authorized to download the resource

Handbooks & Protocols (1)
For purification of mitochondria from eukaryotic cells and tissues
Show details
Kit Handbooks (1)
For purification of mitochondria from eukaryotic cells and tissues
Show details
fragment fix placeholder

Customers who bought these products also bought

  • Cat No./ID: 217004
    /qiagenstorefront/be//sample-technologies/rna/mirneasy-mini-kit/

    miRNeasy Mini Kit (50)

    For 50 preps: 50 RNeasy Mini Spin Columns, Collection Tubes (1.5 ml and 2 ml), QIAzol Lysis Reagent, RNase-Free Reagents and Buffers
    €362.00
  • Cat No./ID: 206143
    /qiagenstorefront/be//pcr/end-point-pcr-enzymes-and-kits/qiagen-multiplex-pcr-kit/

    QIAGEN Multiplex PCR Kit (100)

    For 100 x 50 µl multiplex PCR reactions: 2x QIAGEN Multiplex PCR Master Mix (providing a final concentration of 3 mM MgCl2, 3 x 0.85 ml), 5x Q-Solution (1 x 2.0 ml), RNase-Free Water (2 x 1.7 ml)
    €249.00