- Parallel expression in three systems with a single construct
- Avoids the need for time-consuming subcloning
- High-level expression
- Tightly regulated expression
- Enhanced stability of cytotoxic constructs
Vectors in the His-Strep pQE-TriSystem Vector Set contain promoters for E. coli, insect-, and mammalian-cell expression systems. Proteins expressed from His-Strep pQE-TriSystem vectors carry a 6xHis- and a Strep-tag, enabling sequential purification on Ni-NTA and Strep-Tactin matrices to give ultrapure (>98% pure) protein. Both vectors encode a C-terminal tag that ensures only full-length protein is purified.