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Investigator STAR Lyse&Prep Kit

For automated purification of DNA from 1–96 samples

  • Purification from forensic and human identity samples
  • Efficient yields from trace casework samples
  • Designed for efficient and safe use in high-throughput laboratories
  • Contains all reagents for lysis, binding, washing and elution of samples
The Investigator STAR Lyse&Prep Kit enables automated purification of genomic DNA from 1–96 samples, such as swabs, casework or crime-scene samples and blood on the STAR Q SP/AS instrument and the Hamilton STARline instruments. The kit format is specially designed for high-throughput applications accelerating the laboratory workflow, and produces high quantities of high-quality DNA for downstream processing.
Cat No./ID: 931447
Investigator STAR Lyse&Prep Kit (400)
For 400 preps of 300 µl each from casework and reference samples: Buffer ATL, Buffer QSL3, Buffer QSW1, Buffer QSW2, Bead Suspension G, Buffer ATE, Proteinase K, Carrier RNA, Q-Card, Handbook
The Investigator STAR Lyse&Prep Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease.
Performance
The Investigator STAR Lyse&Prep Kit enables highly efficient purification of DNA from forensic casework samples in a high-throughput setup. The kit includes sufficient reagents for 400 samples, and includes multiple bottles of buffers and enzymes measured to enable utilization of one bottle per sample prep batch of up to 96 samples. This ensures efficient use of reagents and minimal storage of opened reagents, reducing any risk of contamination. Input volumes can be adjusted to accommodate a larger lysis volume for larger samples, and use of the Investigator Lyse&Spin Basket Kit baskets.
Principle
The Investigator STAR Lyse&Prep Kit reproducibly automate purification of genomic DNA, encountered in forensic and human identity applications. The purification procedure is efficient, and purified DNA performs well in downstream analyses, such as quantitative PCR and STR analysis. It is designed to ensure safe and reproducible handling of precious or potentially infectious samples, and comprises 4 steps: lyse, bind, wash and elute.

Since the type of samples that can be processed using the Investigator STAR Lyse&Prep Kit can vary greatly, there is also a variety of different pretreatments, optimized for specific sample types. Samples are lysed under denaturing conditions, in the presence of proteinase K and Buffer ATL, in total volumes of either 300 μl (intended for routine use) or 500 μl (facilitating the use of the Investigator Lyse&Spin Basket Kit baskets and also processing of larger samples).

Magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles, in a format designed specifically for the STAR Q SP/AS instrument and the Hamilton STARline instruments. DNA is isolated from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet. The DNA is then efficiently washed and eluted in modified TE buffer (Buffer ATE). The user can choose elution volumes of either 50 or 100 μl.
Applications

The high-quality DNA obtained using the Investigator STAR Lyse&Prep Kit is suitable for direct use in forensic and human identity testing for applications such as:

  • Genotyping, including fingerprinting and paternity analysis
  • DNA purification from trace samples (e.g., crime-scene)
  • DNA extraction from filters used in environmental testing
  • Routine analysis of reference samples
Features
Specifications
Applications STR analysis, (real-time) PCR
Applications STR analysis, (real-time) PCR
Elution volume 50 or 100 µl
Elution volume 50 or 100 µl
Input volume 300 or 500 µl lysate
Input volume 300 or 500 µl lysate
Sample type Wide range of forensic and human-identity sample materials
Sample type Wide range of forensic and human-identity sample materials
Technology Magnetic-particle technology
Technology Magnetic-particle technology
Yield <3 µg
Yield <3 µg
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