Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
QIAcube HT enables automated mid- to high-throughput nucleic acid purification in 96-well format using silica membrane technology. A variety of QIAcube HT Purification Kits lets users quickly and easily purify DNA, RNA and miRNA from almost any type of sample – including cells, tissues and stool samples, as well as from bacteria and viruses in animal samples. Automated protocols and dedicated accessories and consumables increase reliability and convenience and save valuable time. The system provides the same high-quality results as other trusted QIAGEN purification technologies.
Explore the virtual demo to learn more about QIAcube HT.
The QIAcube HT is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
QIAcube HT provides fast and reliable automated high-throughput nucleic acid purification for use in a variety of sensitive downstream applications. Researchers can easily increase their sample purification throughput and expect the same high-quality results as from other trusted QIAGEN nucleic acid purification solutions.
DNA purificationAutomated purification of total DNA from blood, cells, and tissue samples is quick, easy, and reliable with QIAcube HT and the dedicated QIAamp 96 DNA QIAcube HT Kit. Fresh or frozen whole blood containing common anticoagulants can be processed (see figure Analysis of genomic DNA purified from human blood). QIAcube HT lets users increase sample purification throughput without having to compromise on quality or reliability, providing high yields of pure DNA that perform well in downstream analyses (see figures Purification of genomic DNA from tissues and High performance in downstream assays).
RNA purificationRNA purification on QIAcube HT is simple and reliable with the RNeasy 96 QIAcube HT Kit and provides linear sample recovery with no significant well-to-well variation across a 96-well plate (see figures Linear recovery of total RNA from tissues and Reproducible 96-well RNA purification). This economical, easy-to-use system provides the same results as other trusted QIAGEN purification technologies (see figure Automated purification of high-quality RNA from tissues).
DNA and RNA from animal samplesQIAcube HT enables reliable purification of nucleic acids from undiluted animal whole blood with minimal risk of silica membrane clogging. Automated processing of samples in 96-well format is precise and repeatable between different purification runs performed on different days, providing not only convenience but also peace of mind (see figure Highly repeatable viral RNA isolation). Co-purification of viral RNA and bacterial DNA is easily automated with QIAcube HT and dedicated cador Pathogen QIAcube HT Kits, and provides the same high-quality nucleic acids as manual processing with the QIAamp cador Pathogen Mini Kit (see figure Co-isolation of viral RNA and bacterial DNA).
DNA from complex food samplesThe DNeasy mericon 96 QIAcube HT Kit has been specially adapted for rapid mid- to high-throughput purification of DNA from a variety of raw and processed food matrices on the QIAcube HT instrument. A modified cetyltrimethylammonium bromide (CTAB) extraction procedure allows DNA extraction from all food samples in radically shorter time compared with conventional CTAB protocols (see figure Efficient and rapid DNA extraction from complex food samples). Now users can prepare the same high-quality DNA as from the manual DNeasy mericon Food Kit with a quick and simple automated procedure (see figure Purification of DNA from complex food samples).
Automated purification on the QIAcube HT is quick and cost-effective and delivers high-quality nucleic acids from 24–96 samples per run. Dedicated QIAcube HT Kits enable purification of nucleic acids from a diverse range of starting materials, including cells, tissues, bacteria, viruses and food material. The QIAcube HT is extremely easy to use, and the intuitive software lets users start a run with just a few mouse clicks.
Five dedicated QIAcube HT purification kits are currently available. The QIAamp 96 DNA QIAcube HT Kit enables automated purification of total DNA from animal blood, cells, and tissue. The QIAamp 96 Virus QIAcube HT Kit provides purification of viral RNA and DNA from a broad range of human sample types including fresh or frozen tissues, blood and other body fluids. The RNeasy 96 QIAcube HT Kit is designed for automated purification of total RNA or total RNA, including miRNA, from animal and human cells and tissue. The cador Pathogen 96 DNA QIAcube HT Kit is for automated purification of viral RNA and DNA and bacterial DNA from animal samples. The DNeasy mericon 96 QIAcube HT Kit is for automated purification of total nucleic acids from a range of raw and processed food sample types. All the plastic consumables required for sample purification runs are conveniently included in the QIAcube HT Plasticware package. Predesigned protocols and instrument run files are easily downloaded from the "Resources" tab on the QIAcube HT catalog page. This maximizes convenience and lets users get up and running quickly in the laboratory.
QIAcube HT is a compact, benchtop instrument with a very small footprint. It fits easily onto most lab benches and can be readily moved into biosafety level laboratories. QIAcube HT comes with a laptop computer for added flexibility.
Advanced instrument safety features, including a translucent hood, help to protect precious samples from environmental contamination. The HEPA filter maintains positive clean air pressure on the worktable underneath the hood and protects precious samples from potential airborne contamination. The UV light provides efficient worktable decontamination and helps to prevent cross-contamination. QIAcube HT ejects used tips through the tip disposal chute into a disposable plastic box located at the side of the instrument. This external tip ejection prevents waste accumulation in the clean workspace. The tip disposal box holder keeps the box positioned correctly under the tip chute. The disposal box also comes with a secure-fitting lid to make waste disposal simple and safe.
QIAcube HT provides automated, high-throughput nucleic acid purification for 24–96 samples per run, in increments of 8 samples. The instrument uses a vacuum-driven system to perform the simple and fast bind–wash–elute procedure during sample purification. The vacuum chamber is composed of two separate compartments. This configuration is one of many measures that minimizes sample cross-contamination during purification.
All materials needed for sample purification are placed onto the instrument worktable prior to the run. A 96-well sample block contains the starting sample material that will be lysed and transferred to the filter plate during the run. Easy-to-fill disposable troughs contain all necessary buffers and reagents for sample binding, washing, and elution. Pipet tips are positioned on the right side of the worktable.
Liquid-handling tasks are performed by the 8-channel pipetting head, which provides fast transfer of liquids during the run. The software tracks tip use and internally updates tip availability as the run proceeds. QIAcube HT optimizes the use of plasticware with a smart and economical tip re-use strategy. After washing, the pipetting head moves the 96-well filter plate to the elution compartment, where purified nucleic acids are eluted into the elution microtubes. The included 8-well strip caps securely close the elution microtubes for sample storage.
QIAcube HT Software provides a user-friendly interface for operating the QIAcube HT instrument. The software wizard simulates the instrument worktable and makes it easy to set up and start a run with just a few mouse clicks. The software automatically calculates reagent volumes according to the number of samples, and fully documents every run. The software also allows you to import bar code databases, manage user access, and integrate Laboratory Information Management Systems (LIMS).
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