GeneRead DNAseq Targeted Panels V2
For targeted enrichment of biologically and clinically relevant panels using any of 15 laboratory-verified primer sets prior to NGS
GeneRead DNAseq Targeted Panels V2 are the simplest tools for analyzing the genetic variants of a focused panel of genes via next-generation sequencing. Each panel consists of multiplex PCR primer sets to amplify exonic regions of a thoroughly researched panel of biologically and clinically relevant and disease-focused genes. GeneRead DNAseq Targeted Panels V2 can also be customized to include genes or other genomic regions tailored to your specific NGS project needs. Our high-quality primer design and targeted enrichment chemistry provide high design coverage, specificity, and uniformity, which are essential for detecting low-frequency variants in your precious samples.
Performance
The performance of GeneRead DNAseq Targeted Panels V2 is assessed by 3 criteria: design coverage, specificity, and coverage uniformity. Greater than 95% of exonic regions are covered by the panel primer design, enhancing potential variant discovery. The high specificity of the system maximizes efficient use of sequencing capacity, as more than 95% of sequencing reads align to target regions. Finally, the high coverage uniformity of the system, with more than 90% of targeted bases covered at >20% median sequencing depth, ensures high-quality variant calls.
Principle
The GeneRead DNAseq Targeted Panel System employs overlapping primer sets across the exonic portions of a gene or group of genes to maximize target coverage (see Multiplex PCR-enabled target enrichment of genomic regions of interest). Overlapping primer sets are divided into an appropriate number of pools to maximize specificity. Following amplification and purification, enriched regions from each sample are pooled together, yielding one library preparation for each sample.
Procedure
GeneRead DNAseq Targeted Panels V2 are part of a total workflow for targeted next-generation sequencing (see GeneRead DNAseq Targeted Panels V2 workflow). Simply extract DNA from your samples (the QIAamp DNA Mini Kit, the QIAamp DNA FFPE Tissue Kit or the GeneRead DNA FFPE Kit are recommended), quantify and qualify your DNA sample with the GeneRead DNA QuantiMIZE system, and then use the GeneRead DNAseq Targeted Panels V2 in combination with GeneRead DNAseq Panel PCR Kit V2 to perform targeted enrichment using multiplex PCR. Once targets have been enriched, construct the NGS library and use the GeneRead DNAseq Library Quant Array to quantify and perform quality control. Perform NGS. Multiple samples can be analyzed with a single NGS run. Use the Multiplexing Capacity Calculator (see the Resources tab) to determine the optimal number of samples and coverage depth for each NGS run. Analyze your data using the QIAGEN NGS Data Analysis Web Portal or CLC Bio pipelines, and interpret detected variants using the Ingenuity Variant Analysis platform.
Applications
GeneRead DNAseq Targeted Panels V2 are highly suited for enriching target genes specific for various cancers, inherited diseases, neurological diseases, cardiomyopathy, developmental disorders, metabolic disorders and many other areas of biomedical research to determine genetic variants through NGS.
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