For isolating viral or bacterial total nucleic acids from waste water and stool samples
Optimized to isolate viral or bacterial DNA and RNA from waste water, stool, biosolids and gut material
Inhibitor Removal Technology eliminates inhibitory substances, including polysaccharides, lipids and heme for nucleic acids that are ready to use in PCR, qPCR, RT-qPCR, RNA-Seq, Sanger sequencing or NGS
Optional bead beating protoocol available with Glass Bead Tubes for optimal lysis of solid samples and bacterial cells and without bead tubes for isolation of viral nucleic acids from liquid samples
The Allprep PowerViral DNA/RNA Kit is designed for fast and easy purification of viral and bacterial total nucleic acids from samples high in PCR inhibitors; including waste water, stool, biosolids, and gut material. Patented Inhibitor Removal Technology (IRT) ensures complete removal of the inhibitory substances often contained in these materials, such as undigested plant material in the gut or heme compounds from lysed red blood cells, abundant in stool. The result is DNA and RNA from virus and bacteria that is ready to use in the most demanding downstream applications. Nucleic acids are eluted in RNase-Free Water and ready for PCR, cDNA synthesis, RT-qPCR, RNA-seq, Sanger sequencing or NGS.
For stool samples or samples with a solid matrix, lysis is achieved using 0.1 mm Glass Bead Tubes in combination with a strong chemical lysis buffer that ensures efficient extraction of tough microorganisms in the bead beating step. For virus extraction from water samples and samples that do not require dispersion, the bead beating step may be skipped and virus extracted through chemical lysis only.
The Allprep PowerViral DNA/RNA Kit was previously sold by MO BIO as the PowerViral Environmental DNA/RNA Kit.
Buffers and reagents for DNA fragmentation (including end repair and A-addition), ligation and library amplification; for use with Illumina instruments; includes a plate containing 96 adapters with different barcodes (pierceable foil seal, allowing usage of defined parts of plate)