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qBiomarker Array PreAMP Mix

For loci-specific preamplification prior to real-time PCR to support ultrasensitive cancer mutation detection
  • Nested preamplification improves sensitivity and decreases false positives
  • PCR-based protocol is accessible to most laboratories
  • Integrated, complimentary data analysis simplifies mutation calling
The qBiomarker Array PreAMP Mix enables preamplification of DNA from small amounts of intact or degraded DNA prior to analysis with qBiomarker Somatic Mutation PCR Arrays. The kit contains a mixture of loci-specific primers which, together with the qBiomarker PreAMP PCR Mastermix, uses multiplex PCR preamplification to amplify loci-specific DNA target templates with minimal bias for analysis with qBiomarker Somatic Mutation PCR Arrays or Assays.
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Available Products
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Product Product no. Cat. no. List price:
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-001A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-002A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-003A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-004A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-006A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-007A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-008A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-009A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-011A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-013A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-014A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-020A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-021A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-023A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-025A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-026A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-027A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-028A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
show details
337041 SMBH-029A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-032B 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-035A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-037A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-038A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-045A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-046A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-802A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-804A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-806A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-807A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-808A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-809A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-810A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-811A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-812A 133,00 €
48 µl PreAMP Mix for a pathway- or disease-related set of loci — enough for 12 reactions
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337041 SMBH-999A 133,00 €
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Principle

For successful somatic mutation detection, experimental methods must detect the DNA molecules with mutations of interest in samples that may contain a vast excess of DNA molecules with the wildtype sequence at the same locus. Based off of this principle, there are two factors that affect these experiments: the inherent percentage of mutant DNA in a sample, and the sensitivity of the method. In the case of cancer, a specific mutation may be found in a small percentage of the tumor, which is a characteristic of tumor heterogeneity. Additionally, different types of samples and sample sources affect this ratio of mutant to wildtype DNA as well as the number of DNA molecules that can be assayed. A method’s sensitivity directly reflects how few mutant DNA molecules can be detected within a given amount of wildtype DNA.

Sample types common in cancer research, including formalin-fixed paraffin embedded (FFPE) samples, serum samples, fine needle biopsies, and sorted cells frequently contain only small amounts of amplifiable DNA. Genomic DNA in FFPE samples is fragmented, for example, while DNA in serum samples is heavily diluted. In the case of fine needle biopsies or sorted cells, these samples have small numbers of cells that will yield limited amounts of genomic DNA. Amplifying genomic DNA enables more accurate detection of the mutations in DNA from these sample types.

The qBiomarker PreAMP system uses nested PCR preamplification to improve specificity and decrease false positives. In a 12-cycle PCR reaction, the loci of interest are amplified and are ready to be analyzed with qBiomarker Somatic Mutation PCR Assays and Arrays. The qBiomarker Array PreAMP Mixes contain primers for loci that correspond to the cataloged qBiomarker Somatic Mutation PCR Arrays. Used in combination with the qBiomarker PreAMP Synthesis Kit, this mix produces amplified products specific for the array to be used.

Procedure
DNA is first isolated from FFPE, serum, or small samples using the appropriate QIAamp or DNeasy kit. The qBiomarker PreAMP Synthesis Kit and qBiomarker Array PreAMP Mix are then used to preamplify the DNA using locus-specific primers. qBiomarker Somatic Mutation Arrays are used for mutation detection, and CT values are uploaded into the GeneGlobe Data Analysis tool for data analysis and mutation calls.
Applications

The qBiomarker Array PreAMP Mix is highly suited for preamplification of genomic DNA from samples with low amounts of amplifiable DNA for exclusive use with qBiomarker Somatic Mutation PCR Arrays. Recommended sample types include:

  • FFPE samples
  • Plasma, serum, urine, or other cell-free body fluids
  • Laser capture microdissection samples
  • Fine needle biopsies
  • FACS-sorted cells

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Handbooks & Protocols (2)
For screening disease-focused mutation panels by PCR
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For real-time PCR-based, pathway- or disease-focused, somatic mutation profiling
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Instrument Technical Documents (1)
For screening disease-focused mutation panels by PCR
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Kit Handbooks (1)
For real-time PCR-based, pathway- or disease-focused, somatic mutation profiling
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