cador Pathogen 96 QIAcube HT Kit
For automated high-throughput isolation of viral RNA and DNA and bacterial DNA from animal samples
The cador Pathogen 96 QIAcube HT Kit enables simple, automated purification of viral RNA and DNA and bacterial DNA from a wide range of animal samples on the QIAcube HT system. Using proven QIAamp silica-membrane technology in a convenient 96-well format, contaminants and inhibitors are removed to yield high-quality pathogen nucleic acids that are ready for downstream analysis.
The cador Pathogen 96 QIAcube HT Kit provides fast and reliable automated purification of viral RNA and DNA and bacterial DNA from a range of animal samples — including whole blood, serum, plasma, body fluids, swabs, washes, tissue, and feces. High-quality nucleic acids are ready for use in a variety of sensitive downstream applications.
Purification of nucleic acids from undiluted animal whole blood is quick and simple, with minimal risk of silica membrane clogging. Automated processing of samples in 96-well format is precise and repeatable between different purification runs performed on different days, providing not only convenience but also peace of mind (see figure Highly repeatable viral RNA isolation).
The QIAcube HT platform with the cador Pathogen QIAcube HT Kit allows parallel purification of different nucleic acids from a range of sample types using just one universal instrument protocol. Co-purification of viral RNA and bacterial DNA is simple and reliable and provides the same high-quality nucleic acids as manual processing with the QIAamp cador Pathogen Mini Kit (see figure Co-isolation of viral RNA and bacterial DNA). Compared with a kit from another supplier, the cador Pathogen 96 QIAcube HT Kit displays high performance, and succeeds even when the other kit falls short (see figure High performance protocol).
The cador Pathogen 96 QIAcube HT Kit enables automated purification of pathogen nucleic acids — viral RNA and DNA and bacterial DNA — from up to 200 μl of animal whole blood, serum, plasma, swabs, or washes using the QIAcube HT instrument. By applying the pretreatments described in the cador Pathogen 96 QIAcube HT Handbook, tissue and fecal samples can also be processed. The procedure yields high-quality DNA and RNA that perform well in PCR, RT-PCR, and other downstream applications.
The cador Pathogen 96 QIAcube HT Kit combines the selective binding properties of a silica-based membrane with a high-throughput 96-well format, and is designed for fully automated, simultaneous processing of 24–96 samples on the QIAcube HT instrument.
Samples are lysed under highly denaturing conditions at room temperature in the presence of proteinase K and Buffer VXL, which together ensure the inactivation of nucleases. Adding Buffer ACB adjusts the binding conditions for the co-purification of DNA and RNA. The lysate is then transferred to a QIAamp 96 plate. As vacuum is applied, DNA selectively binds to the QIAamp membrane as contaminants pass through. Three efficient wash steps remove the remaining contaminants and enzyme inhibitors, and nucleic acids are eluted in Buffer AVE.
Most common fluid samples can be processed directly using the main protocol, which involves the lysis, binding, wash, and elution steps. However, depending on the starting material and the target pathogen, one of the pretreatment protocols may be needed (see the table "Pretreatments for the cador Pathogen 96 QIAcube HT Kit protocol"). After pretreatment, the samples undergo the same procedure as for fluid samples, enabling automated parallel processing on the QIAcube HT.
The cador Pathogen 96 QIAcube HT Kit yields pure, high-quality viral RNA, viral DNA, and bacterial DNA that is suitable for a wide range of downstream applications, including real-time PCR and RT-PCR for:
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