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Strep-tag Antibody

For highly sensitive and specific detection of Strep-tagged proteins
  • Excellent results in dot- and western-blotting procedures
  • Highly sensitive and specific detection
Monoclonal mouse Strep-tag antibodies are used to detect recombinant proteins carrying the short Strep-tag affinity tag epitope with high specificity and sensitivity. Like all QIAGEN mouse monoclonal antibodies, they are prepared under serum-free conditions — guaranteeing absence of viruses, mycoplasma, and contaminating immunoglobulins.
Cat No./ID: 34850
Strep-tag Antibody (100 μg)
€563.00
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Mouse monoclonal antibody that recognizes the Strep-tag II epitope; lyophilized, for 1000 ml working solution
The Strep-tag Antibody is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
1
Two-step affinity purification procedure.
Performance
The Strep-tag Antibody allows highly sensitive detection of proteins (see figures Highly sensitive detection of proteins carrying a Strep-tag and Ultrapure protein in two steps)
Principle

The Two-Step Affinity Purification System, which is based on the proven 6xHis tag and the short Strep-tag II, enables simple and highly efficient purification of ultrapure proteins in a fast and standardized procedure. Recombinant proteins carrying both tags are purified sequentially on Ni-NTA and Strep-Tactin matrices (see figure Ultrapure protein in two steps). The two simple affinity purifications provide fully active, full-length, ultrapure protein, suitable for any downstream application.

Procedure

Recombinant proteins that carry two small affinity tags (the 6xHis tag and Strep-tag II) are efficiently expressed in E. coli, insect, or mammalian cells using pQE-TriSystem His·Strep vectors. After cell lysis and clearing of the lysate, proteins are initially purified using an immobilized-metal affinity chromatography procedure that is based on the proven 6xHis-tag-Ni-NTA interaction. After elution from the Ni-NTA matrix using imidazole, recombinant proteins (which also carry the Strep-tag II epitope) are loaded directly onto a Strep-Tactin matrix. No buffer exchange is required. Protein is eluted from the Strep-Tactin matrix using either biotin or desthiobiotin. This two-step affinity purification delivers ultrapure (>98% pure) protein (see figure Two-step affinity purification procedure). The order of purifications can be reversed (i.e., Strep-Tactin followed by Ni- NTA purification). Proteins can be detected with high specificity and sensitivity using mouse monoclonal Strep-tag or Anti·His antibodies.

Applications

The two-step affinity purification system, using the Strep-tag Antibody for detection, is highly suited for applications where high purity is at a premium or is difficult to achieve. The standardized purification procedure also increases throughput by eliminating the need for protein-specific purification protocol development and optimization. The ultrahigh purity and convenience provided by the two-step affinity purification system make it the method of choice for:

Structural and functional analyses
Expression in eukaryotic systems
Features
Specifications
Applications Western blot, dot blot, ELISA, immunoprecipitation, immunohistochemistry
Detection Secondary antibody required
Epitope detected SAWSHPQFEK
Sensitivity in Western blots (chemiluminescent detection) 1 ng
Substrate for blot detection Dependent on secondary antibody
Substrates for assay procedure Dependent on secondary antibody
Tag Strep-tag
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