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Custom Mix-n-Match Multi-Analyte ELISArray Kits

For the simultaneous detection of a custom panel of up to 12 cytokines or chemokines of your choice in multiple biological samples using an ELISA

  • Detection of a custom panel of up to 12 cytokines or chemokines
  • High sensitivity and linearity
  • Screened and verified capture and detection antibodies
  • Uses standard ELISA equipment

Custom Mix-n-Match Multi-Analyte ELISArray Kits allow you to choose up to 12 cytokines and chemokines for simple, simultaneous detection in multiple samples. You also have the choice to select fewer cytokines and increase the number of samples per plate. The optimized combination of high-quality capture and detection antibodies and colorimetric detection reagents provide each Custom Mix-n-Match ELISArray Kit with the best sensitivity and linearity available. Arranged in a 96-well plate, Custom Mix-n-Match ELISArray Kits use a standard ELISA protocol and provide the same quality and performance as the catalogued Multi-Analyte ELISArray Kits.

Choose cytokines and chemokines for your Custom Mix-n-Match Multi-Analyte ELISArray Kit from this excel file.


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Product Product no. Cat. no. List price:
Custom Mix-n-Match Multi-Analyte ELISArray Kit
1 plate (twelve 8-well strips) precoated with capture antibodies, and reagents
336111 Varies
593,00 €
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The Custom Mix-n-Match Multi-Analyte ELISArray Kits is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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ELISArray protocol.

Multi-Analyte ELISArray Kits follow a traditional ELISA protocol that can be completed in any laboratory with a microplate reader.
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Multi-Analyte ELISArray plate layout.

Each Multi-Analyte ELISArray contains twelve 8-well strips (represented by colored columns) coated with a specific capture antibody for a cytokine or chemokine. Six samples can be analyzed at one time, in addition to controls.
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Patterns of Th1/Th2 cytokine induction.

Th1/Th2 cytokines were induced by human peripheral blood mononuclear cells in response to PMA (50 µg/ml) and ionomycin (1 µg/ml). Time-dependent patterns of induced cytokines and relative cytokine amounts were profiled and plotted.

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Optimized ELISA conditions enable the same development or incubation time.

Similar standard curves are observed for all optimized cytokine and chemokine assays in the Multi-Analyte ELISArray Kits. All standard curves are virtually superimposable indicating that all 12 assays provide similar linearity and sensitivity under the same standardized conditions and development or incubation times.

Performance

A Multi-Analyte ELISArray Kit was used to profile multiple cytokines secreted by stimulated human peripheral blood mononuclear cells (PBMCs). The time-dependent (0, 6, 18, 24, and 48 hours) patterns of 8 Th1/Th2 cytokine inductions by human PBMCs were montored in response to PMA (50 µg/ml) and ionomycin (1 µg/ml) (see figure "Patterns of Th1/Th2 cytokine induction"). The relative amount of each of the 8 cytokines was also profiled using the Multi-Analyte ELISArray Kit.

Large amounts of IL-2 and IFNγ, and to a lesser extent TNFα, are induced throughout, and apparently beyond, the time course tested. Only more moderate amounts of IL-4 and IL-5 are induced, reaching a maximum at approximately 24 hours of stimulation. The even lower levels of IL-10 and IL-13 that are secreted also reach a maximum at 24 hours and appear to decrease again at later times. IL-12 is not detectable after 48 hours of constant stimulation.

Principle

The Custom Mix-n-Match Multi-Analyte ELISArray Kit profiles a customized panel of up to 12 cytokines or chemokines in 6 cell supernatant (media), serum, or plasma samples using one simple protocol and one standardized development time (see figure "Multi-Analyte ELISArray plate layout"). Custom Mix-n-Match Multi-Analyte ELISArray Kits provide researchers with a rapid assessment of the level of multiple proteins of their choice (cytokines and chemokines) in multiple samples.

Commercially available capture and detection antibodies are screened to achieve the highest possible sensitivity and linearity for each cytokine or chemokine assay. All assays are optimized under uniform conditions to allow simultaneous detection without compromising performance (see figure "Optimized ELISA conditions enable the same development or incubation time").

Procedure

Custom Mix-n-Match Multi-Analyte ELISArray Kits use a standard sandwich ELISA protocol (see flowchart "ELISArray Protocol"). There is no requirement for special equipment; kits are compatible with a standard ELISA plate reader.

Applications

Custom Mix-n-Match Multi-Analyte ELISArray Kits are highly suited for research into autoimmune and immune disorders, cancer, immunology, infectious diseases, and many other areas of biological and medical research. They can be used in any application typically analyzed using individual ELISA kits.

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Kit Handbooks (2)
For the simultaneous detection of up to 12 cytokines or chemokines in multiple samples
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For the simultaneous detection of up to 12 cytokines or chemokines in multiple samples
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