Qproteome Total Glycoprotein Kit
For isolation of glycosylated proteins
The kit contains lectin-resin filled spin columns, buffers, and reagents for the isolation of glycoproteins from cell lysate or serum samples.
Spin Column Adapter Rings are required for automation of this kit on the QIAcube.
The Qproteome Total Glycoprotein Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
The Qproteome Glycoprotein Kit allows highly specific fractionation of proteins based on their glycan moieties (see figure "Highly specific glycoprotein fractionation").
Glycosylated proteins in the sample bind to lectins immobilized on the resin in the spin columns. After washing, proteins are eluted in two stages by sequential addition of buffers containing sugars which compete with bound proteins for the lectin binding sites.
The sample is diluted and applied to the spin column (see figure "Glycoprotein fractionation procedure"). After a short incubation, non-glycosylated proteins are removed by centrifugation. Glycosylated proteins are eluted by application of elution buffer and centrifugation.
Glycosylation plays a vital role in a wide range of cellular processes such as cell adhesion and signaling, stabilization of protein structure and function, protein trafficking and sorting, and oncogenesis. Several diseases (e.g., rheumatoid arthritis, muscular dystrophy) may be caused by a defect in protein glycosylation. The Qproteome Glycoprotein Fractionation Kit offers highly specific separation of glycoproteins according to the structure of their glycan moieties, and permits profiling of glycoproteins in cells grown under different conditions. Purified glycoproteins are suitable for direct analysis using blotting procedures, 2D-PAGE, or mass spectrometry methods.
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