miScript miRNA Inhibitors
For studies on miRNA function and gene regulation using miRNA inhibition
miScript miRNA Inhibitors are chemically synthesized, single-stranded, modified RNAs which specifically inhibit endogenous miRNA function after transfection into cells. miScript miRNA Inhibitors are available at cell-culture grade (>90% purity) or animal grade (HPLC purified). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale. For flexible screening, miScript miRNA Inhibitor Plates enable researchers to order any predesigned or custom-designed miScript miRNA Inhibitors in their choice of layout in 96-well or 384-well plates. miScript miRNA Mimics can be used for similar experiments.
miScript miRNA Inhibitors have been designed and modified to ensure efficient inhibition of endogenous miRNA function (see figure miScript miRNA Inhibitor counteracts miRNA-induced silencing). Real-time PCR analysis detected a significant decrease in miRNA level after transfection of miScript miRNA Inhibitor (see figure "Transfection of miRNA inhibitor decreases endogenous miRNA level"). Time-course experiments indicate that miScript miRNA Inhibitors are effective for at least 96 hours following transfection (see figure "miRNA inhibitor effective after 96 hours").
Transfection of inhibitors, followed by downstream gene expression analysis or phenotypic analysis, is performed to elucidate the targets and roles of particular miRNAs. These experiments enable study of the biological effects of misregulation of individual miRNAs as well as confirmation of specific genes as targets of individual miRNAs.
Increased gene expression after transfection of inhibitor provides evidence that the miRNA under study is involved in regulation of that gene. Similar studies can be performed using miScript miRNA Mimics. Typically, such experiments involve transfection of mimic or inhibitor, or alternatively cotransfection with a vector construct which carries the miRNA-binding site fused to a reporter gene. Downstream analysis can include reporter assays, real-time PCR, microarray analysis, or protein analysis.
Highly flexible solutions offered by miScript miRNA Inhibitors are available for every human, mouse, rat, and virus miRNA in the current version of the microRNA database, miRBase. QIAGEN's GeneGlobe database is continually updated to ensure that mimic and inhibitor designs match the most up-to-date version of miRBase. This ensures that you are always working with the most accurate and current miRNA sequence information possible. If the miRNA of interest is not available in miRBase, an inhibitor for your human, mouse, rat, or virus miRNA can be custom synthesized by visiting QIAGEN's custom miScript product design page.
Flexible formats enable either low-throughput analysis using small numbers of inhibitors or high-throughput screening using hundreds of inhibitors. miScript miRNA Inhibitors are available at cell-culture-grade (>90% purity provided by proprietary synthesis and purification processes). For in vivo applications, HPLC-purified, animal-grade miScript miRNA Inhibitors can be ordered at the 20 nmol scale (provided in tubes). miScript miRNA Inhibitors are also available phosphorothioate modified at the 20 nmol scale for in vivo applications. Phosphorothioate modification protects the inhibitor from exonuclease activity, increasesing the stability of the molecule. miScript miRNA Inhibitors can easily be ordered at GeneGlobe. For medium- to high-throughput applications, 96-well plates, or 384-well plates are available to suit your experimental requirements.
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