For automated purification of total RNA from cells and tissues on the QIAcube

  • Fast setup with preloaded rotor adapters
  • Standardized results and streamlined workflow
  • Helps to eliminate loading errors
  • Kit provides everything needed for automated processing
  • Automated purification from up to 12 samples per run

The RNeasy Mini QIAcube Kit is a highly convenient format of the RNeasy Mini Kit, dedicated for use on the QIAcube. The RNeasy Mini QIAcube Kit enables purification of consistent yields of high-quality RNA from cells and tissues.

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Product Cat. no. List price:
RNeasy Mini QIAcube Kit (240)
For 240 RNA minipreps: RNeasy Mini Rotor Adapters (preloaded with spin columns and elution tubes); RNase-free reagents and buffers
74116
1.483,00 €
The RNeasy Mini QIAcube Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Significant time savings.|
The y-axis shows a comparison of the average percentage of hands-on time required for sample prep using manual processing, using the QIAcube, and using the QIAcube with dedicated kits.|
Performance
The RNeasy Mini QIAcube Kit, including rotor adapters preloaded with RNeasy spin columns and elution tubes, delivers greater convenience and time savings (see figure "Significant time savings with dedicated QIAcube Kits").
Principle
The RNeasy Mini QIAcube Kit includes rotor adapters that are preloaded with RNeasy spin columns and elution tubes, increasing ease of use and minimizing user errors. Waste is reduced as the content of the dedicated kit is tailored to purification on the QIAcube. Tubes required for the manual procedure are not included. Using stringent guanidine-isothiocyanate lysis with the purity of silica-membrane purification, the RNeasy Mini QIAcube Kit enables standardized RNA purification from up to 1 x 107 cells or 30 mg tissue.
Procedure

The QIAcube uses advanced technology to process QIAGEN spin columns, enabling seamless integration of automated, low-throughput sample prep into laboratory workflows. All steps in the purification procedure are fully automated — and up to 12 samples can be processed per run. The QIAcube together with the dedicated RNeasy Mini QIAcube Kit provides fast, easy, and convenient RNA purification.

Applications

The RNeasy Mini QIAcube Kit provides high-quality RNA suitable for downstream applications, such as:

Quantitative real-time RT-PCR
Array analysis
Feature
Specifications
Applications PCR, qPCR, real-time RT-PCR, microarray
Elution volume 30–100 µl
Format Spin column, preloaded into rotor adapters
Main sample type Tissue, cells
Processing Automated (QIAcube)
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Total RNA
Sample amount 0.5–30 mg
Technology Silica technology
Yield Varies

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