For gel extraction of up to 5 μg DNA fragments (70 bp to 4 kb) in low elution volumes
  • Very small elution volumes
  • Fast procedure and easy handling
  • High, reproducible recoveries
  • Gel loading dye for convenient sample analysis

The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription. The MinElute Gel Extraction Kit can be automated on the QIAcube.
For optimal results it is recommended to use this product together with QIAvac 24 Plus.

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Product Cat. no. List price:
MinElute Gel Extraction Kit (50)
50 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28604
120,00 €
MinElute Gel Extraction Kit (250)
250 MinElute Spin Columns, Buffers, Collection Tubes (2 ml)
28606
554,00 €
The MinElute Gel Extraction Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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MinElute procedure.|Higher DNA concentrations.|GelPilot Loading Dye.|MinElute membrane assembly.|pH Indicator Dye.|Spin column handling options — D.|Spin column handling options — E.|Spin column handling options — C.|Spin column handling options — B.|Spin column handling options — A.|
The simple bind-wash-elute procedure ensures greater convenience.
|A 500 bp and a 1000 bp fragment purified using the MinElute Gel Extraction Kit and three different silica-based DNA purification kits from the indicated suppliers. Two microliters of each eluate was loaded onto a 1.5% agarose gel. M: markers.|GelPilot Loading Dye contains three tracking dyes to facilitate optimization of DNA resolution.
|MinElute spin column in cross section, showing the unique membrane assembly (utility model pending).
|pH indicator dye in the solubilization and binding buffer allows easy visual determination of optimal pH for DNA adsorption (pH ≤7.5). An incorrect binding-mixture pH can occur if the agarose gel electrophoresis buffer was frequently used or incorrectly prepared. In this case, the pH can be easily adjusted by addition of 10 µl 3 M sodium acetate, pH 5.0.
|QIAvac 24 plus.
|QIAcube.
|Manifold with luer connectors.
|QIAvac 24.
|Microcentrifuge.
|
Performance

The MinElute Gel Extraction Kit removes nucleotides, enzymes, salts, agarose, ethidium bromide, and other impurities from DNA samples, delivering highly concentrated DNA suitable for a variety of downstream applications (see figure "Higher DNA concentrations").

The MinElute QIAquick Gel Extraction Kit provides spin columns for gel extraction. Using a microcentrifuge or vacuum manifold, high concentration DNA of 70 bp – 4 kb is quickly purified. (DNA fragments from 4 kb – 10 kb should be purified using the QIAquick Gel Extraction Kit and DNA fragments smaller than 70 bp or larger than 10 kb should be extracted with the QIAEX II Gel Extraction System.)

Principle

MinElute Kits contain a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. Specialized binding buffers are optimized for specific applications and promote selective adsorption of DNA molecules within particular size ranges.

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure "GelPilot Loading Dye").

Procedure

The MinElute system uses a simple bind-wash-elute procedure (see flowchart "MinElute procedure"). Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure "pH Indicator Dye"), and the mixture is applied to the MinElute spin column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as the QIAvac 24 Plus with QIAvac Luer Adapters. The MinElute Gel Extraction Kit, in addition to other QIAGEN spin-column-based kits, can be fully automated on the QIAcube, enabling increased productivity and standardization of results (see figures "Spin column handling options A, B, C, D, and E").

Applications

DNA fragments purified with the MinElute or QIAquick System are ready for direct use in all applications, including:

Sequencing, including next generation sequencing 
Microarray analysis
Ligation and transformation
Restriction digestion
Labeling
Feature
Specifications
Binding capacity 5 µg
Elution volume 10 µl
Format Tube
Fragment size 70 bp – 4 kb
Processing Manual
Recovery: oligonucleotides dsDNA Recovery: dsDNA fragments
Sample type: applications DNA: PCR reactions
Technology Gel extraction

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Kit Handbooks
2
For MinElute PCR Purification Kit, MinElute Gel Extraction Kit, MinElute Reaction Cleanup Kit
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MinElute PCR Purification Kit
低溶出量でのPCR産物(70 bp~ 4 kb)精製用

MinElute Gel Extraction Kit
低溶出量でのDNAフラグメント(70 bp~ 4 kb)のゲル抽出

MinElute Reaction Cleanup Kit
酵素反応液からのDNA(70 bp~ 4 kb)クリーンアップ用
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References
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