artus HAV TM RT-PCR Kit v2.0 RUO

For quantitative detection of hepatitis A virus RNA using real-time PCR
  • High reliability using the internal control
  • Sensitive detection of as few as 0.1 IU/µl
  • Accurate quantitation using the 4 standards supplied

The artus HAV TM RT-PCR Kit v2.0 is a ready-to-use molecular detection kit for real-time PCR on ABI PRISM 7000, 7700, and 7900HT Sequence Detection Systems. The kit provides all necessary reagents optimized for rapid and sensitive detection of HAV RNA from human serum and plasma samples with highly accurate quantification.

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artus HAV TM RT-PCR Kit v2.0 (24) RUO
For 24 reactions: 2 Masters, 4 Quantitation Standards, Internal Control, Water (PCR grade)
4516103
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For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Reliable quantitation of HAV load.|
Detection of the quantitation standards (HAV TM QS 1–4) on the ABI PRISM 7900HT SDS by measurement of the FAM fluorescence signal. NTC: no template control.|
Performance

To ensure sensitivity, the artus HAV TM RT-PCR Kit v2.0 has been optimized to detect low numbers of HAV RNA. The analytical sensitivity of the kit is 0.2 IU/μl in the PCR on the ABI PRISM 7000 SDS and 0.1 IU/μl in the PCR on the ABI PRISM 7700 SDS and the ABI PRISM 7900HT SDS.

For reliable specificity, testing of the artus HAV TM RT-PCR Kit v2.0 was carried out using various HAV strains, including all relevant genotypes (IA, IB, II, IIIA, VII), and related pathogens.

Performance of artus HAV TM RT-PCR Kit v2.0
Kitartus HAV TM RT-PCR Kit v2.0
Sample types Serum or plasma
Analytical sensitivity 0.2 IU/µl (ABI PRISM 7000 SDS); 0.1 IU/µl (ABI PRISM 7700 SDS and 7900HT SDS)
 Specificity  HAV, including genotypes IA, IB, II, IIIA, and VII

Principle

The artus HAV TM RT-PCR Kit v2.0 is based on the amplification and simultaneous detection of a highly conserved region within the HAV genome using real-time PCR. The kit provides high levels of specificity and sensitivity.

The artus HAV TM RT-PCR Kit v2.0 provides 4 HAV quantitation standards (see figure "Reliable quantitation of HAV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kit contains a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical HAV RT-PCR is not reduced.

Specifications of artus HAV TM RT-PCR Kit v2.0
Kitartus HAV TM RT-PCR Kit v2.0
Sample types Serum or plasma
Amplicon 90 bp region of the HAV genome

Procedure

The artus HAV TM RT-PCR Kit v2.0 provides all necessary reagents optimized for reliable HAV RNA detection and quantitation. Simply add template RNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

For RNA purification, we recommend using the QIAamp UltraSens Virus Kit, QIAamp Viral RNA Mini Kit, QIAamp DSP Virus Kit, or QIAamp DNA Stool Mini Kit.

Applications
The artus HAV TM RT-PCR Kit v2.0 allows the rapid and sensitive detection of HAV RNA from serum and plasma. The kit is available for use on ABI PRISM 7000, 7700, and 7900HT Sequence Detection Systems.
Feature
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep Depending on the starting material and sensitivity needs: QIAamp Ultrasens Virus Kit, QIAamp Viral RNA Mini Kit, QIAamp DSP Virus Kit, QIAamp DNA Stool Mini Kit
RUO/CE/ASR RUO
Sample type Plasma, serum, fecal suspensions
Thermal cycler ABI PRISM 7000, 7700, 7900HT SDS
What detected Hepatitis A Virus RNA

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Kit Handbooks
1
For use with the ABI PRISM 7000, 7700 and 7900HT Sequence Detection Systems
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