GeneRead Sequencing Q Kit
For preparation of DNA sequencing for next-generation sequencing (NGS) applications using the QIAGEN GeneReader instrument
Next-generation sequencing (NGS) is a driving force for numerous new and exciting applications, including cancer research, stem cell research, metagenomics, population genetics and medical research. The QIAGEN GeneRead Sequencing Q Kit consists of easy-to-use buffers and reagents that only need to be mixed together before loading onto the GeneReader instrument, saving time and preventing handling errors. Optimized enzyme and buffer compositions ensure high quality sequencing performance, perfect for research applications that require precise data insight. Streamlined GeneRead sequencing protocols enable straightforward automation of sequencing-by-synthesis technology on the GeneReader.
The GeneRead Sequencing Q Kits are for Research Use Only. Not for use in diagnostic procedures.
The GeneRead Sequencing Q Kit provides high quality reagents that are easy to mix and install on the GeneReader for a sequencing run. The GeneReader workflow includes the following processes: sequencing primer hybridization, flow cell preparation, reagents preparation, flow cell loading and run start (refer to the QIAGEN GeneReader User Manual for additional information). These procedures are described in detail starting on page 12 of the GeneReader User Manual. GeneReader sequencing chemistry is briefly described in the Procedure section below. It consists of a unique terminator-dNTP sequencing-by-synthesis paradigm that ensures highly accurate and cost-effective NGS runs.
Reagents and disposable flow cells are available in two kit sizes: GeneRead Sequencing Q Kit (1) and GeneRead Sequencing Q Kit (4). Each kit contains three boxes as described below. Wash buffers are available in the separate GeneRead Sequencing Buffer Q Kit (16).
The GeneRead Sequencing Q Kit (1) allows the user to perform a single flow cell run. The GeneRead Sequencing Q Kit (4) allows the user to perform runs with the following 3 setup options:
Note: Flow cells can be run via staggered loading into the QIAGEN GeneReader after a run has started.
After DNA library construction, DNA clonally amplified using the GeneRead QIAcube is immobilized via direct bead-slide interaction and exposed to a DNA sequencing primer to produce a high-density array on a GeneReader Flow Cell. To read out the sequence of each of the beads, the array of fragments is first subjected to reagents containing uniquely engineered DNA bases that include a removable fluorescent dye and an end cap. These bases attach themselves to the end of the growing strand of DNA in accordance with the base on the complementary strand. The array is scanned by a high-resolution electronic camera and the fluorescent output of each of the four dye colors at each array position is measured and recorded. The color indicates which base (A, C, G or T) was incorporated onto the DNA fragment from the previous step. Finally, the array is exposed to cleavage chemistry to break off the fluorescent dye and end cap that will then allow additional bases to be added. This cycle is then repeated on the GeneReader.
For use with the QIAGEN GeneReader instrument.
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