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ipsogen RUNX1-RUNX1T1 Kit

For quantitative detection of RUNX1-RUNX1T1 (or AML1-ETO) fusion gene transcripts using real-time PCR
  • Sensitive quantitative assay
  • Accurate quantification relative to ABL control gene expression
  • EAC standardized assay for reproducible results
  • Optimal quality control and ISO 13485 certification
  • Easy workflow with ready-to-use solutions
The ipsogen RUNX1-RUNX1T1 Kit is intended for research use only and is not for use in diagnostic procedures. The kit is for real-time PCR on the Rotor-Gene Q and other real-time PCR instruments. The ipsogen RUNX1-RUNX1T1 Kit provides reagents optimized for reliable and sensitive detection and quantification of RUNX1-RUNX1T1 (or AML1-ETO) fusion gene transcripts in bone marrow or peripheral blood samples, relative to ABL control gene expression.
Cat No./ID: 675013
ipsogen RUNX1-RUNX1T1 Kit (24)
For 24 reactions: ABL Control Gene Standards, RUNX1-RUNX1T1 Fusion Gene Standards, Primers and Probe Mix ABL, Primers and Probe Mix RUNX1-RUNX1T1 Fusion Gene
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
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Sensitive detection of RUNX1-RUNX1T1 and ABL standards.
Detection of [A] RUNX1-RUNX1T1 standards (F1 to F5) with 101, 102, 103, 105, and 106 copies/5 μl and [B] ABL standards (C1, C2, C3) with 103, 104, and 105 copies/5 μl.
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RUNX1-RUNX1T1 fusion gene transcript.
Performance
To ensure highest sensitivity, the ipsogen RUNX1-RUNX1T1 Kit has been optimized to detect ABL and RUNX1-RUNX1T1 fusion gene transcripts over a wide dynamic range (see figure "Sensitive detection of RUNX1-RUNX1T1 and ABL standards").
Principle
The ipsogen RUNX1-RUNX1T1 Kit is a ready-to-use kit for detection and quantification of RUNX1-RUNX1T1 fusion gene transcripts using real-time PCR. The kit is based on the amplification and detection of specific RUNX1-RUNX1T1 fusion gene transcripts, relative to ABL control gene expression in total RNA (see figures "RUNX1-RUNX1T1 fusion gene transcript" and "ABL control gene transcript", and provides high levels of specificity, sensitivity, and reproducibility.
Each ipsogen RUNX1-RUNX1T1 Kit provides 3 standard dilutions for the ABL gene and 5 standard dilutions for the RUNX1-RUNX1T1 fusion gene. Primers and probe sets are designed according to Europe Against Cancer (EAC) recommendations.
Procedure
The first step is reverse transcription of total RNA in a sample into cDNA. The second step is the amplification of cDNA by real-time PCR. Using the ipsogen RUNX1-RUNX1T1 Kit allows detection and quantification of RUNX1-RUNX1T1 fusion gene and ABL transcripts. Simply start the reaction using the optimized protocols described in the kit handbook.
Applications
The ipsogen RUNX1-RUNX1T1 Kit enables sensitive and reliable detection and quantification of RUNX1-RUNX1T1 fusion gene transcripts relative to ABL control gene expression for research use only.
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