RespiFinder RG Panel RUO
For detection and differentiation of 16 RNA viruses, 2 DNA viruses, and 4 bacteria within 4.5 hours
The RespiFinder RG Panel is a qualitative multiplex PCR test to detect and differentiate 16 RNA viruses, 2 DNA viruses, and 4 bacteria within 4.5 hours. (The RespiFinder RG Panel detects 16 RNA viruses but does not differentiate rhinovirus from enterovirus.)
For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
The RespiFinder RG Panel is based on the SmartFinder technology, a technology that allows a highly complex analysis of multiple targets in real-time PCR. The RespiFinder RG Panel contains 23 different MultiFinder probe sets combined with 13 fluorescent labeled SMART (single-tube multiplex amplification in real time) probes that enable the detection of 22 different pathogens, plus an internal control (see table "Pathogens and corresponding target genes of the RespiFinder RG Panel").
The RespiFinder RG Panel uses the Rotor-Gene Q instrument for the detection of the pathogen(s) nucleic acids.
An internal control (IC) is included in the assay to discriminate between true negative samples and false negative samples due to nucleic acid degradation, PCR inhibition, or test failure.
The input sample is total nucleic acids extracted and purified from nasopharyngeal swabs using the QIAamp MinElute Virus Spin Kit.
The panel contains primers, probes, enzymes, and reagents for detection and differentiation of pathogen nucleic acids. Pre-amplification and hybridization are carried out on a standard block-based thermal cycler, followed by ligation and PCR on the Rotor-Gene Q instrument. Melting curve analysis on the Rotor-Gene Q enables differentiation of the pathogen nucleic acid targets.