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RespiFinder RG Panel CE

For detection and differentiation of viruses and bacteria that can cause respiratory tract infections
  • Compliance with EU IVD Directive 98/79/EC
  • Analysis of multiple targets in one reaction using SmartFinder technology 
  • Detection and differentiation of 22 pathogen nucleic acids
  • High reliability using the internal control
The RespiFinder RG Panel is a qualitative multiplex PCR test to detect and differentiate 16 RNA viruses, 2 DNA viruses and 4 bacteria.
Cat No./ID: 4692163
RespiFinder RG Panel (25) CE
For 25 reactions: Pre-amplification Master Mix, Internal Control, Pre-amplification Primermix, Probemixes, Ligase Enzyme, Taq Polymerase, and Buffers

The RespiFinder RG Panel CE is intended for in vitro diagnostic use in Europe.


Performance
Principle
The RespiFinder RG Panel is based on the SmartFinder technology, a technology that allows a highly complex analysis of multiple targets using real-time PCR. The RespiFinder RG Panel contains 23 different MultiFinder probe sets combined with 13 fluorescent-labeled SMART (single-tube multiplex amplification in real time) probes that enable the detection of 22 different pathogens, plus an internal control (see table "Pathogens and corresponding target genes of the RespiFinder RG Panel").
Pathogens and corresponding target genes of the RespiFinder RG Panel
Target Gene
Control
Internal control (IC) MS2 phage lysis protein gene
 Viruses  
 Adenovirus  Hexon gene (H)
 Bocavirus  Noncapsid gene (NP1)
 Corona 229E  Nucleocapsid protein gene (NP)
 Corona HKU1  Nucleocapsid phosphoprotein gene (N)
 Corona NL63  Nucleocapsid protein gene (NP)
 Corona OC43  Nucleocapsid protein gene (NP)
 hMPV  Nucleocapsid protein gene (NP)
 Influenza A  Matrix protein gene (M1)
 Influenza B  Matrix protein gene (M1)
 Influenza A H1N1v  Neuraminidase gene
 Parainfluenza 1  Hemagglutinin-neuraminidase gene (HN)
 Parainfluenza 2  Hemagglutinin-neuraminidase gene (HN)
 Parainfluenza 3  Hemagglutinin-neuraminidase gene (HN)
 Parainfluenza 4  Major nucleocapsid protein gene (N)
 Rhinovirus/Enterovirus  5' untranslated region polyprotein gene (PP)
 RSV-A  Major nucleocapsid protein gene (N)
 RSV-B  Major nucleocapsid protein gene (N)
 Bacteria  
 Bordetella pertussis  Insertion sequence 481 (IS481)
 Chlamydophila pneumoniae
 Major outer membrane gene (OmpA)
 Legionella pneumophila  Macrophage inhibitor potentiator gene (Mip)
 Mycoplasma pneumoniae  Cytadhesin protein gene (P1)
The reaction starts with a pre-amplification that combines a reverse transcription step with a PCR step to amplify the target DNA/cDNA. Subsequently, a part of the pre-amplification reaction is transferred to 2 PCR tubes. Two separate SmartFinder reactions are performed, comprising a probe hybridization step and a probe ligation and amplification step. The detection is performed using melting curve analysis.
The RespiFinder RG Panel uses the Rotor-Gene Q MDx instrument for the detection of the pathogen(s) nucleic acids.
An internal control (IC) is included in the assay to discriminate between true negative samples and false-negative samples due to nucleic acid degradation, PCR inhibition, or test failure.
Procedure
The input sample is total nucleic acids extracted and purified from nasopharyngeal swabs using the QIAamp MinElute Virus Spin Kit.
The RespiFinder RG Panel contains primers, probes, enzymes and reagents for detection and differentiation of pathogen nucleic acids. Pre-amplification and hybridization are carried out on a standard block-based thermal cycler, followed by ligation and PCR on the Rotor-Gene Q MDx instrument. Melting curve analysis on Rotor-Gene Q MDx enables differentiation of the pathogen nucleic acid targets.
Applications
The RespiFinder RG Panel is for in vitro diagnostic use for detection and differentiation of viruses and bacteria that can cause respiratory tract infections in humans.

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