An aid to the detection of Mycobacterium tuberculosis (TB) infection (1)
Accurate and unaffected by prior BCG vaccination (2–4)
Efficient, with results after one patient visit (1)
Objective results (1)
Proven performance in immunosuppressed patients (5–8)
QuantiFERON-TB Gold is an in vitro diagnotic test that aids in the detection of infection with M. tuberculosis. QFT is an interferon γ (IFNγ) release assay, also known as an IGRA, which measures the cell-mediated response to specific TB antigens in whole blood.
For 56 tests/kit (28 per full plate) based on 4-point curve when running standards in triplicate, includes: Microplate Strips; Human IFNγ Standard, lyophilized; Green Diluent; Conjugate 100x Concentrate, lyophilized; Wash Buffer 20x Concentrate; Enzyme Substrate Solution; Enzyme Stopping Solution
For 560 tests (28 per full plate) depending on optimization of run, includes: Microplate Strips; Human IFNγ Standard, lyophilized; Green Diluent; Conjugate 100x Concentrate, lyophilized; Wash Buffer 20x Concentrate; Enzyme Substrate Solution; Enzyme Stopping Solution
The QuantiFERON-TB Gold (QFT) Kit is intended for in vitro diagnostic use.
Diagnosing or excluding TB disease, and assessing the probability of latent TB infection (LTBI), requires a combination of epidemiological, historical, medical, and diagnostic findings that should be taken into account when interpreting a QFT result.
QFT Package Insert, March 2013, US05990301L.
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Cheallaigh, et al. (2013) PlOs One 8, e53330.
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QuantiFERON-TB Gold interpretation flowchart 1
Interpretation flowchart when Nil and TB Antigen tubes are used.
QuantiFERON-TB Gold interpretation flowchart 2
Interpretation flowchart when Nil, TB Antigen, and Mitogen tubes are used.
QuantiFERON-TB Gold tubes
QuantiFERON-TB Gold tubes are available for normal altitudes and high altitudes
Tuberculosis (TB) remains a significant threat to humanity, and therefore identifying TB infection is essential. A person infected with Mycobacterium tuberculosis, but who shows no symptoms is regarded as having latent TB infection. Global organizations acknowledge that to fight TB effectively, accurate identification and treatment of latent TB infection, as well as active TB disease, are vital (9).
QuantiFERON-TB Gold (QFT) is a modern alternative to the 110 year old tuberculin skin test (or Mantoux test) and is the most studied IGRA, with over 800 published peer-reviewed publications. The test is highly accurate and unaffected by Bacillus Calmette-Guérin (BCG) vaccination (2–4).
QFT is an interferon-gamma (IFNγ) release assay, commonly known as an IGRA, and measures the cell-mediated immune response to specific TB antigens in whole blood. Patients may be tested in a single patient visit, with objective results, eliminating the need for two-step testing (1). The test has proven performance in immunosuppressed patients (5–8).
By reducing false positive results seen with TST, QFT reduces the costs associated with unnecessary chest X-rays, chemoprophylaxis, toxic side effects, lost productivity, and labor costs resulting from two-step TST testing. Studies show that QFT can reduce the cost of maintaining healthcare worker screening programs by up to 32% (10).