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artus VZV PCR Kits CE

For quantitative detection of varicella-zoster virus specific DNA using real-time PCR
  • Complete CE-IVD-compliant workflow (QIAsymphony RGQ)
  • High reliability using the internal control
  • Highly sensitive detection of as few as 19.2 copies/ml (QS-RGQ kit)
  • Accurate quantitation using the 4 standards supplied
artus VZV PCR Kits are ready-to-use molecular detection kits for real-time PCR. The kits provide all necessary reagents optimized for reliable VZV DNA detection and quantitation for in vitro diagnostic use. artus VZV LC, RG, and TM PCR Kits use manual or automated sample preparation and manual assay setup techniques. The artus VZV RG PCR Kit is also available as EASYartus VZV RG PCR Kits, which include the EZ1 DSP Virus Kit for automated viral nucleic acid purification. The artus VZV QS-RGQ Kit is part of the QIAsymphony RGQ, the complete automated system from sample to pathogen detection, and is not for use with manual assay setup.
Cat No./ID: 4502063
artus VZV LC PCR Kit (24) CE
For 24 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4502065
artus VZV LC PCR Kit (96) CE
For 96 reactions on the LightCycler 1.1/1.2/1.5/2.0 Instruments: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4502163
artus VZV TM PCR Kit (24) CE
For 24 reactions on the ABI PRISM 7000, 7700, 7900HT SDS: Master, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4502263
artus VZV RG PCR Kit (24) CE
For 24 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4502265
artus VZV RG PCR Kit (96) CE
For 96 reactions on Rotor-Gene Q instruments: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)
Cat No./ID: 4502363
artus VZV QS-RGQ Kit (24) CE
For 24 reactions on the QIAsymphony RGQ: Master, Mg Solution, 4 Quantitation Standards, Internal Control, Water (PCR grade)

artus VZV PCR Kits CE are intended for in vitro diagnostic use in Europe.


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Reliable quantitation of VZV load.
Detection of the quantitation standards (VZV RG QS 1–4) on the Rotor-Gene Q using the artus VZV RG PCR Kit. NTC: no template control.
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Varicella-zoster virus.
2
Highly sensitive detection of VZV DNA.
Probit analysis: VZV (QIAsymphony RGQ). Analytical sensitivity of the artus VZV QS-RGQ Kit with the QIAsymphony RGQ system.
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CE-IVD-marked.
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In vitro diagnostic medical device.
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Integrated QIAsymphony RGQ system for VZV detection.
Performance

To ensure highest sensitivity, artus VZV Kits have been optimized to detect low numbers of VZV DNA. The analytical sensitivity of the artus VZV QS-RGQ Kit is 19.2 copies/ml in consideration of the purification from plasma and assay setup using the QIAsymphony RGQ (see figure "Highly sensitive detection of VZV DNA"). 

Performance characteristics of artus VZV RG PCR Kit and artus VZV QS-RGQ Kit
Kit  artus VZV RG PCR Kitartus VZV QS-RGQ Kit
Validated sample types  Human cerebral spinal fluid (CSF) Human cerebral spinal fluid (CSF) or EDTA plasma
Analytical sensitivity  0.136 copies/μl in the PCR 80.7 copies/ml (CSF)
19.2 copies/ml (plasma)
in consideration of the purification
 Linear range  n.d.  500 to 1.00 x 108 copies/ml (CSF)
127 to 6.92 x 106 copies/ml (plasma)
n.d.: not determined.

Principle

artus VZV Kits are based on the amplification and simultaneous detection of a specific region of the VZV genome using real-time PCR. The kits provide high levels of specificity, sensitivity (see figure "Highly sensitive detection of VZV DNA"), and reproducibility.

Each artus VZV Kit provides 4 VZV quantitation standards (see figure "Reliable quantitation of VZV load"). Use of the standards enables accurate quantitation of viral load. In addition, the kits contain a second heterologous amplification system to identify possible PCR inhibition. This is detected as an internal control (IC) in a different fluorescence channel from the analytical PCR. The detection limit of the analytical VZV PCR is not reduced.

Specifications of artus VZV RG PCR Kit and artus VZV QS-RGQ Kit
Kitartus VZV RG PCR Kit artus VZV QS-RGQ Kit
Validated sample types Human cerebral spinal fluid (CSF) Human cerebral spinal fluid (CSF) or EDTA plasma
Amplicon 82 bp region of ORF38 protein (ORF38) gene 82 bp region of ORF38 protein (ORF38) gene

Procedure

artus VZV PCR Kits provide all necessary reagents optimized for reliable VZV DNA detection and quantitation. Simply add template DNA to the ready-to-use PCR master mix, and start the reaction on the appropriate real-time cycler using the optimized cycling program described in the kit handbook.

Common temperature profiles for multiple assays in one run

The temperature profile of the artus VZV LC PCR, RG PCR, and QS-RGQ Kits corresponds to the profiles of the artus LC PCR, RG PCR, and QS-RGQ Kits for CMV, EBV, and HSV-1/2, as well as for BK virus (RG and QS-RGQ only). Therefore, these PCR assays can be performed and analyzed in a single run.

Complete automated system from sample to VZV detection

The QIAsymphony RGQ workflow solution for VZV detection comprises the QIAsymphony SP for sample preparation, the QIAsymphony AS for assay setup, and the artus VZV QS-RGQ Kit on the Rotor-Gene Q. The system enables reliable pathogen detection with a complete CE-IVD-compliant workflow (see figure "Integrated QIAsymphony RGQ system for VZV detection").

Recommendations for viral DNA purification when using the artus VZV LC, RG, or TM PCR Kits

For DNA purification, the EZ1 DSP Virus Kit is to be used or, depending on the sample type and artus VZV PCR Kit, the QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit. The artus VZV RG PCR Kit is also available as CE-IVD-marked EASYartus VZV RG PCR Kits, which include the EZ1 DSP Virus Kit for automated viral nucleic acid purification.

Applications

artus VZV PCR Kits enable rapid and sensitive detection of VZV DNA from human samples with highly accurate quantification. 

The artus VZV QS-RGQ Kit is designed to be used with the QIAsymphony RGQ system, providing a complete CE-IVD-compliant workflow from sample to VZV DNA detection and quantitation.

Features
Specifications
Quantitative/qualitative Quantitative
Recommended sample prep QIAamp DNA Mini Kit or QIAamp UltraSens Virus Kit
RUO/CE/ASR CE
Sample type Serum, plasma, cerebrospinal fluid (CSF), swabs
Thermal cycler Rotor-Gene Q; ABI PRISM 7000, 7700, 7900HT SDS, LightCycler
What detected Varicella-zoster virus DNA

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Protocol Files (1)
For use with QIAsymphony SP/AS instruments (software version 4.0)
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Instrument Technical Documents (1)
Information document for software version 4.0
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Handbooks & Protocols (1)
For use with Rotor-Gene Q Instruments
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