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Investigator ESSplex SE QS Kit

For multiplex amplification of the European Standard Set (ESS), SE33 and Amelogenin, with an innovative Quality Sensor

  • Integrated Quality Sensor for better decision making and data interpretation
  • Faster results with a PCR reaction speed of around 60 minutes
  • Simple analysis with minimal overlapping allele panels and an extended ladder
  • High sensitivity and inhibitor resistance
The Investigator ESSplex SE QS Kit amplifies the full suite of 16 STR markers in the European Standard Set (ESS), including SE33 and the gender-specific Amelogenin, as well as a unique performance control called Quality Sensor (QS). The Quality Sensor provides additional, valuable data for your sample quality control and PCR performance check. By taking advantage of QIAGEN´s fast-cycling technologies, the kit enables PCR amplification in just 60 minutes. Highly sensitive, with rapid and reliable results from trace DNA, the Investigator ESSplex SE QS Kit is suitable for all forensic applications and paternity testing.
Cat No./ID: 381575
Investigator ESSplex SE QS Kit (100)
€2,093.00
Add To Cart
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water
Cat No./ID: 381577
Investigator ESSplex SE QS Kit (400)
€7,603.00
Add To Cart
Primer Mix, Fast Reaction Mix including Taq DNA Polymerase, Control DNA, Allelic Ladder ESSplex SE QS, DNA size standard 550 (BTO) and Nuclease-Free Water
The Investigator ESSplex SE QS Kit is intended for molecular biology applications in forensic, human identity and paternity testing. This product is not intended for diagnosis, prevention or treatment of a disease.
Performance
The Invesigator ESSplex SE QS Kit utilizes fast-cycling technology to provide reliable results in approximately 60 minutes.
The novel Fast Reaction Mix 2.0 is based on proven QIAGEN multiplex PCR technology that has been further optimized for direct amplification. Assay performance tests in the presence of increasing concentrations of inhibitors showed superior inhibitor resistance. This means reliable, high-quality results.
The Investigator ESSplex SE QS Kit was validated and evaluated using the GeneAmp 9700 thermal cycler (in standard mode) and the Applied Biosystems 3500 Genetic Analyzer.
Principle
The Investigator ESSplex SE QS Kit uses QIAGEN's fast-cycling PCR technology for the simultaneous amplification of 16 STR markers and Amelogenin, as recommended by the European Network of Forensic Science Institutes (ENFSI) and the European DNA Profiling Group (EDNAP) as the European Standard Set of loci (ESS).

The Investigator ESSplex SE QS Kit Primer Mix contains the new innovative performance control Quality Sensor (QS1 and QS2). It provides additional information on sample quality and PCR success. The Quality Sensor, being simultaneously amplified with the STR markers, shows you if you are dealing with:

  • Successful PCR amplification
  • Failed PCR amplification
  • Absence of DNA
  • Degraded DNA
  • Inhibitors


The additional information obtained with the Quality Sensor will help you streamline your sample analysis by showing you where to focus your analytical efforts, and save you time and money by eliminating the need for unnecessary PCR re-runs.
Procedure
The 16 STR markers, Amelogenin and the Quality Sensor are amplified simultaneously in a single PCR, and labeled with one of the dyes listed in Table 1 "Investigator ESSplex SE QS Kit dyes and corresponding markers". 
The loci then undergo capillary electrophoresis. The results can be analyzed using dedicated software, such as the Applied Biosystems GeneMapper ID-X. See the table "Investigator ESSplex SE QS Kit technical specifications" for more information.
 
Table 1. Investigator ESSplex SE QS Kit dyes and corresponding markers
Dye Marker            
6-FAM QS1 Amelogenin TH01 D3S1358 vWA D21S11 QS2
BTG D163539 D1S1656 D19S433 SE33      
BTY D10S1248 D22S1045 D12S391 D8S1179 D2S1338    
BTR D2S441 D18S51 FGA        

Table 2. Investigator ESSplex SE QS Kit technical specifications
Category Details
Volume per PCR 25 µl
Matrix BT5
Fluorescence labels 6-FAM, BTG, BTY, BTR, BTO
Genetic analyzers ABI PRISMR 3100/3100-Avant, Applied Biosystems 3130/3130xl or
Applied Biosystems 3500/3500xL Genetic Analyzers
Software for analysis  Applied Biosystems GeneMapper ID, GeneMapper ID-X, or Genotyper Software 

Applications

The Investigator ESSplex SE QS Kit enables simultaneous amplification of the European Standard Set (ESS), including SE33, and is highly suitable for:

  • Criminal database and forensic casework samples
  • Paternity testing
  • Population genetics studies
  • Other human identity testing applications

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For further information on which version of the v9 template files is suitable for your needs, please refer to the Template File Info Note that can also be found in the ''Template File'' section of the product resources.
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