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virotype PRRSV NA/EU RT-PCR Reagent

For the safe and simultaneous identification of RNA from the North American (NA) and European (EU) genotypes of porcine reproductive and respiratory syndrome virus (PRRSV).
  • Simultaneous RNA identification of the NA and EU genotypes of PRRSV
  • Easy-to-use single reaction mix containing controls
  • RT-PCR run time of less than 2 hours
The virotype PRRSV NA/EU RT-PCR Rgt permits the simultaneous identification of RNA from the North American (NA) and European (EU) genotypes of porcine reproductive and respiratory syndrome virus (PRRSV). The reagent contains primer-probe sets for specific amplification of a highly conserved region of the PRRSV genome. The reagent also contains an internal control to identify possible PCR inhibition, and an external positive control.

For further information, please contact us.
Cat No./ID: 282315
virotype PRRSV NA/EU RT-PCR Rgt (96)
For 96 reactions: Master Mix, Positive Control
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.
Principle
This is a real-time RT-PCR reagent for the identification of RNA from North American (NA) and European (EU) genotypes of PRRSV. RT-PCR is based on the amplification of specific regions of the pathogen genome. It is a sensitive and specific tool for pathogen identification.

Real-time RT-PCR involves the reverse transcription of viral RNA and subsequent amplification of the cDNA by PCR. The amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows the identification of the accumulating product without the need to re-open the reaction tubes afterwards.

Procedure
Prior to real-time RT-PCR, viral RNA must be extracted from the starting material. QIAGEN offers a range of products for RNA extraction from animal samples.
Applications
The virotype PRRSV NA/EU RT-PCR Rgt is designed to identify North American (NA) and European (EU) RNA genotypes of PRRSV in biological samples using polymerase chain reaction (PCR).

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