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pigtype Toxoplasma Ab

For detection of antibodies to Toxoplasma gondii in samples from swine and other mammalian species
  • Highly sensitive and specific indirect ELISA for Toxoplasma gondii
  • Suitable for serum, plasma. and meat juice samples
  • User-friendly test protocol with ready-to-use colored reagents
  • Results in less than 2 hours with potential overnight protocol 
pigtype Toxoplasma Ab is a sensitive and specific ELISA for detecting antibodies to Toxoplasma gondii in serum, plasma, and meat juice samples from pigs and wild boar and in serum and plasma samples from sheep, goat, cattle, fox, dog, and cat.
 
Cat No./ID: 273401
pigtype Toxoplasma Ab (1)
For 96 reactions: 1 Test Plate, Wash Buffer, Sample Diluent, anti-IgG-HRP Conjugate, TMB Substrate Solution, Positive Control, Negative Control, Stop Solution 
Cat No./ID: 273403
pigtype Toxoplasma Ab (5)
For 480 reactions: 5 Test Plates, Wash Buffer, Sample Diluent, anti-IgG-HRP Conjugate, TMB Substrate Solution, Positive Control, Negative Control, Stop Solution 

pigtype Toxoplasma Ab is for the detection of antibodies to Toxoplasma gondii in serum, plasma, and meat juice samples from pigs and wild boar and in serum and plasma samples from other mammalian species such as sheep, goat, cattle, fox, dog, and cat.

pigtype Toxoplasma Ab is for veterinary use only.

In Germany: Registered in accordance with §17c of the German Law on Animal Diseases. Registration No.: FLI-B 564


0
Sensitivity and specificity of pigtype Toxoplasma Ab.
Serum samples of a temporal study with 4 pigs experimentally infected with T. gondii were analyzed. All of the serum samples tested positive 2 weeks-post-infection to the end of the temporal study, providing a high diagnostic sensitivity.
Performance
pigtype Toxoplasma Ab is specific for the detection of antibodies to Toxoplasma gondii. A negative serum population was tested. All of the samples tested negative for Toxoplasma-specific antibodies, providing a high diagnostic specificity for this sample set. In addition, serum samples of a temporal study with 4 pigs experimentally infected with T. gondii were simultaneously analyzed. All of the serum samples tested positive 2 weeks-post-infection to the end of the temporal study, providing a high diagnostic sensitivity (see the figure Sensitivity and specificity of pigtype Toxoplasma Ab).
Principle
pigtype Toxoplasma Ab is an ELISA (enzyme-linked immunosorbent assay) with ready-to-use colored reagents. The test plate is coated with inactivated Toxoplasma antigen. During the sample incubation, Toxoplasma-specific antibodies bind to the immobilized antigen. The anti-IgG-HRP conjugate detects antibodies bound to the antigen. The color reaction is started by adding the substrate solution and stopped after 10 minutes. The optical density (OD) is measured in a spectrophotometer. The OD values correlate with the concentration of anti-Toxoplasma antibodies in the sample.
Procedure
Serum, plasma, and meat juice samples can be tested after dilution.

There are two protocols for this product. They differ in the length of the incubation period. Samples are incubated for one hour or overnight with the test plate and if antibodies to Toxoplasma gondii are present, they will bind to the antigen on the plates. Unbound material is removed by rinsing.

The anti-IgG-HRP conjugate is added. Unbound conjugate is rinsed out.

The color reaction is started by adding the TMB Substrate Solution and stopped after 10 minutes. The optical density (OD) is measured using a spectrophotometer. The ratio sample OD to Positive Control OD is calculated from the OD values obtained with the test sample and the Negative Control, which contains no Toxoplasma-specific antibodies. The values are easy to interpret.
Applications
pigtype Toxoplasma Ab is a sensitive and specific indirect ELISA for detecting antibodies to Toxoplasma gondii in serum, plasma, and meat juice samples from pigs and wild boar and in serum and plasma samples from other mammalian species such as sheep, goat, cattle, fox, dog, and cat.

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