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cador Salmonella PCR Reagent

For the identification of salmonella DNA
  • A ready-to-use system for real-time PCR
  • Highly sensitive and specific identification of salmonella DNA
  • Internal Control to monitor PCR inhibition
  • A full license for PCR without additional costs
The cador Salmonella PCR reagent is a highly sensitive and specific assay to identify salmonella DNA in biological samples.

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Cat No./ID: 288315
cador Salmonella Reagent (96)
For 96 reactions: cador PCR + IC Reagents, cador Salmonella reagents
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention or treatment of a disease. Regulatory requirements vary by country; product may not be available in your geographic area.

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Efficient and precise identification of Salmonella DNA over a wide linear range.
Duplex real-time PCR was carried out using the cador Salmonella PCR Reagent and the Rotor-Gene Q according to the supplied protocol. Salmonella DNA was serially diluted as indicated in the figure. Each concentration was analyzed in triplicate. [A] Amplification plots for salmonella DNA (one replicate is shown for each template dilution). [B] Amplification plots for IC DNA (one replicate is shown for each template dilution). [C] The log template amount was plotted versus mean CT value, demonstrating a high efficiency, linearity, and precision in identification of salmonella DNA. Error bars represent ± 1 SD of 3 replicates.
Performance
The cador Salmonella Reagent contains reagents and enzymes for the specific and efficient amplification of highly conserved regions of the salmonella genome. Inhibition and other malfunctions of PCR are determined by measuring the fluorescence signal in the yellow channel via amplification of the Internal Control (IC), which does not influence the amplification of the analytical PCR for salmonella. An external positive control (salmonella Control DNA, containing the targeted salmonella DNA) is also supplied. salmonella DNA is detected efficiently and precisely over a wide linear range (see the figure Efficient and precise identification of salmonella DNA over a wide linear range).

Several other pathogens that are not intended to be identified using the assay may also be present in the animal sample material. To test for potential cross-reactivity, the cador Salmonella PCR Reagent was used for analysis of other bacterial species. No cross-reactivity was observed (see the Table Analysis of potential cross-reactivity).

Analysis of potential cross-reactivity
Species Subspecies 10,000 copies/reaction 10 copies/reaction
Escherichia E. coli Not detected Not detected
Campylobacter C. jejuni Not detected Not detected
Campylobacter C. coli Not detected Not detected
Campylobacter C. lari Not detected Not detected
Cronobacter C. sakazakii Not detected Not detected
Shigella S. flexneri Not detected Not detected
Shigella S. dysenteriae Not detected Not detected
Listeria L. monocytogenes Not detected Not detected
Staphylococcus S. aureus Not detected Not detected
Bacillus B. cereus Not detected Not detected
Yersinia Y. enterocolica Not detected Not detected
Clostridium C. perfringens Not detected Not detected
Legionella L. erythra Not detected Not detected
Legionella L. pneumophila Not detected Not detected
Escherichia VTEC stx1/ stx2 Not detected Not detected
Principle
Pathogen identification by the polymerase chain reaction (PCR) is based on the amplification of specific regions of the pathogen genome. In real-time PCR, the amplified product is detected using fluorescent dyes. These are usually linked to oligonucleotide probes that bind specifically to the amplified product. Monitoring the fluorescence intensities during the PCR run (i.e., in real time) allows detection of the accumulating product without having to re-open the reaction tubes afterward, which reduces the risk of cross contamination.
 
The cador Salmonella PCR Reagent contains a primer-probe set specific for a highly conserved region of the salmonella genome. The reagent also includes a heterologous amplification system as an internal control to ensure the correct interpretation of negative results, and an external positive control. Real-time PCR detection is carried out on a real-time PCR cycler, such as the Rotor-Gene Q.
Procedure
Bacterial DNA can be manually isolated from samples using the QIAamp cador Pathogen Mini Kit. The isolated DNA is ready for use in real-time PCR with the cador Salmonella PCR Reagent on a real-time PCR cycler, such as the Rotor-Gene Q.
Applications
The cador Salmonella PCR Reagent is designed to identify salmonella DNA in biological samples using polymerase chain reaction (PCR).

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