|
Altitude
|
Up to 2000 m (6500 ft.)
|
|
Elution volume
|
QIAsymphony SP — 30–500 µl (application dependent)
|
|
Features
|
Rotor-Gene Q — dynamic range, 10 orders of magnitude
|
|
Heat dissipation/thermal load
|
Rotor-Gene Q — average, 0.183 kW (632 BTU/hour); peak, 0.458 kW (1578 BTU/hour)
|
|
Humidity
|
QIAsymphony SP/AS — relative humidity of 15–75% (noncondensing); maximum 75% relative humidity for temperatures up to 31°C, decreasing linearly to 50% humidity at 32°C
Rotor-Gene Q — relative humidity of 10–75% (noncondensing)
|
|
Input volume
|
QIAsymphony SP — minimum input volume: 200 µl (application dependent)
|
|
Instrument dimensions
|
QIAsymphony SP/AS — QIAsymphony SP, 128 x 103 x 73 cm; QIAsymphony AS, 59 x 103 x 73 cm; QIAsymphony SP/AS (integrated operation), 185 x 103 x 73 cm
Rotor-Gene Q — width, 37 cm (14.6 in.); height, 28.6 cm (11.3 in.); depth (without cables), 42 cm (16.5 in.); depth (door open), 53.8 cm (21.2 in.)
|
|
Kits or Application Packs designed for this instrument
|
QIAsymphony SP — QIAsymphony DSP Virus/Pathogen Kits and other QIAsymphony Kits; QIAsymphony AS — artus QS-RGQ Kits (not available in all countries), Rotor-Gene Kits and other QIAGEN real-time and end-point PCR kits
Rotor-Gene Q — artus QS-RGQ Kits (not available in all countries), RG SYBR Green PCR Kits; RG SYBR Green RT-PCR Kit; RG Probe PCR Kits; RG Probe RT-PCR Kit; RG Multiplex PCR Kit
|
|
Operating temperature
|
QIAsymphony SP/AS — 15–32°C (59–89.6ºF)
Rotor-Gene Q — 18–30ºC (64–86ºF)
|
|
Optical System
|
Rotor-Gene Q — up to 6 channels spanning UV to infra-red wavelengths; excitation sources: high energy light-emitting diodes; detector: photomultiplier; acquisition time: 4 s; the software allows to create new excitation/detection wavelength combinations
|
|
Overvoltage category
|
II
|
|
Place of operation
|
For indoor use only
|
|
Pollution level
|
QIAsymphony SP/AS — 2
Rotor-Gene Q — 2; Environmental class 3K2 (IEC 60721-3-3)
|
|
Power
|
QIAsymphony SP/AS — 100–240 V AC, 50–60 Hz, 1400 VA, mains supply voltage are not to exceed 10% of nominal supply voltages
Rotor-Gene Q — 100–240 V AC, 50–60 Hz, 520 VA (peak); Power consumption 8 VA (standby); Mains supply voltage fluctuations are not to exceed 10% of the nominal supply voltages; F5A 250 V fuse
|
|
Processing
|
QIAsymphony SP/AS — automated
|
|
Protocols/main application on this instrument
|
QIAsymphony SP — purification of DNA, RNA, and bacterial and viral nucleic acids from a wide range of starting materials
QIAsymphony AS — PCR setup
Rotor-Gene Q — gene expression analysis, microRNA detection, virus detection, SNP genotyping, SNP genotyping, High Resolution Melt analysis (HRM)
|
|
Samples per run (throughput)
|
QIAsymphony SP — 1–96 samples in batches of 24
Rotor-Gene Q — tubes 0.2 ml; strip tubes 0.1 ml (4 tubes); Rotor-Disc 72; Rotor-Disc 100; up to 100 samples per run using a Rotor-Disc 100
|
|
Software
|
Rotor-Gene Q — Rotor-Gene Q software, supplied on the provided installation CD
|
|
Storage temperature
|
QIAsymphony SP/AS — 5–40°C (41ºF to 104ºF) in manufacturer's package
Rotor-Gene Q — 15 to 30ºC (59ºF to 86ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 1K2 (IEC 60721-3-1)
|
|
Technology
|
QIAsymphony SP — QIAGEN magnetic-particles chemistry
Rotor-Gene Q — real-time PCR cycler
|
|
Thermal performance
|
Rotor-Gene Q — temperature range, 35 to 99ºC; temperature accuracy, ±0.5ºC (type, measured 30 seconds after clock start); temperature resolution, ±0.02ºC (smallest programmable increment); temperature uniformity, ±0.02ºC; ramp rate (peak ramp rates, air), >15ºC/s heating, >20ºC/s cooling
|
|
Transportation conditions
|
QIAsymphony SP/AS — –25 to 70°C (–13ºF to 158ºF) in manufacturer's package
Rotor-Gene Q — –25 to 60ºC (–13ºF to 140ºF) in manufacturer’s package; max. 75% relative humidity (noncondensing); environmental class 2K2 (IEC 60721-3-2)
|
|
Typical run time
|
Rotor-Gene Q — 40 cycles in 45 min with the QIAGEN RG Kits (assay dependent)
|
|
Warranty
|
Rotor-Gene Q — 1 year on instrument; lifetime guarantee on excitation LEDs
|
|
Weight
|
QIAsymphony SP, 175 kg; QIAsymphony AS, 90 kg; QIAsymphony SP/AS (integrated operation), 265 kg; Rotor-Gene Q, 12.5 kg (27.6 lb.) (standard configuration)
|