QIAsymphony DSP DNA Kits

For automated purification of DNA from 1–96 samples

  • Purification of DNA from a broad range of sample types
  • Novel, prefilled reagent cartridges for ease of use
  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents

QIAsymphony DSP DNA Kits, in combination with the QIAsymphony SP, enable automated DNA purification from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood for in vitro diagnostic use. Kits are available in mini and midi formats for sample volumes ranging from 200 µl to 1000 µl. For tissue and FFPE tissue protocols, manual sample pretreatment is required. Optimized protocols provide high yields of pure DNA, and flexible elution volumes allow DNA concentration to be easily optimized for each downstream application. Novel, prefilled reagent cartridges reduce setup time and minimize manual handling. Bar code reading enables full tracking of reagents.

Additional applications that require user validation can be found on the "Product Resources" tab.

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Product Cat. no. List price:
QIAsymphony DSP DNA Midi Kit (96)
For 96 preps of 1000 µl each or 144 preps of 400 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
 937255
771,00 €
QIAsymphony DSP DNA Mini Kit (192)
For 192 preps of 200 µl each: Includes 2 reagent cartridges and enzyme racks and accessories.
 937236
842,00 €
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QIAsymphony DSP DNA Kits are intended for in-vitro diagnostic use.


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Prefilled, sealed reagent cartridges.|QIAsymphony DSP DNA procedure.|Schematic of the QIAsymphony SP principle.|
||The QIAsymphony SP processes a sample containing magnetic particles as follows: A magnetic rod protected by a rod cover enters a well containing the sample and attracts the magnetic particles. The magnetic rod cover is positioned above another well and the magnetic particles are released. The QIAsymphony SP uses a magnetic head containing an array of 24 magnetic rods, and can therefore process up to 24 samples simultaneously. Steps 1 and 2 are repeated several times during sample processing.|
Principle

QIAsymphony DSP DNA Kits can be used to purify DNA from human whole blood, buffy coat, tissues and FFPE tissues, as well as viral DNA from human whole blood.

QIAsymphony DSP DNA Kits combine the speed and efficiency of silica-based purification of DNA with the convenient handling of magnetic particles (see figure "Schematic of the QIAsymphony SP principle"). Innovative, ready-to-run reagent cartridges are prefilled with all reagents required for the purification procedure and are automatically opened by the QIAsymphony SP, which helps to minimize the risk of environmental contamination (see figure "Prefilled, sealed reagent cartridges"). Worktable setup is rapid, which saves you valuable time. Simply place up to 2 reagent cartridges in the consumables drawer. The reagent cartridges can be either from the same kit or from different kits, enabling different purification procedures to be performed within the same run of 96 samples. Reagent volumes only for the selected number of samples are used, giving you complete cost control.

Procedure
The purification procedure is designed to ensure safe and reproducible handling of potentially infectious samples, and comprises 4 steps: lyse, bind, wash, and elute (see figure "QIAsymphony DSP DNA procedure"). Optimized protocols provide high quality nucleic acids with efficient removal of proteins, nucleases, and other impurities. Nucleic acids purified with QIAsymphony DSP DNA Kits are ready for direct use in downstream applications such as amplification or other enzymatic reactions. The user can choose between different elution volumes.
Applications

QIAsymphony DSP DNA Kits enable purification of high-quality DNA that is ready for direct use in downstream applications, including sequencing and sensitive detection assays such as quantitative real-time PCR or RT-PCR.

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Application/Protocol Documents for IVD Use
7
(EN) - Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP
For use with software version 4.0
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(EN) - DNA_Blood_1000_V7_DSP
For use with software version 4.0

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(EN) - DNA_Blood_200_V7_DSP
For use with software version 4.0
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(EN) - DNA_Blood_400_V6_DSP
For use with software version 4.0
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(EN) - DNA_Buffy_Coat_200_V7 DSP
For use with software version 4.0
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(EN) - DNA_Buffy_Coat_400_V6_DSP
For use with software version 4.0
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(EN) - VirusBlood200_V5_DSP
For use with software version 4.0
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Application/Protocol Documents for User-Validated Use
1
(EN) - Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP (user-validated for QIAsymphony DSP DNA Mini Kit)
Automated DNA purification from cultured cells and bacterial cultures using the QIAsymphony DSP DNA Mini Kit in combination with the QIAsymphony SP.

The QIAsymphony DSP DNA Mini Kit in combination with the Tissue_LC_200_V7_DSP and Tissue_HC_200_V7_DSP (user-validated) protocols for purification of total DNA from cultured cells and bacterial cultures is intended for molecular biology applications. This combination is not intended for the diagnosis, prevention, or treatment of a disease.

For use with software version 4.0
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