For automated purification of DNA from 1–6 or 1–14 human biological specimens using the EZ1 Advanced or the EZ1 Advanced XL

  • CE-marked in compliance with EU IVD Directive 98/79/EC
  • Rapid purification of high-quality DNA from whole blood samples
  • Flexible purification of 1–6 or 1–14 samples per run
  • Easy protocol setup with credit-card ease of use
  • Standardized processing ensures reproducible results

The EZ1 DSP DNA Blood Kit contains all required reagents and labware for automated purification of DNA from 200 or 350 µl human whole blood using magnetic particle technology. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the EZ1 Advanced or the EZ1 Advanced XL. The CE-IVD-marked system provides exact performance specifications, assuring highly reliable DNA purification.

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Product Cat. no. List price:
EZ1 DSP DNA Blood Kit (48)
For 48 DNA preps: Prefilled Reagent Cartridges, Disposable Tip-Holders, Disposable Filter-Tips, Sample Tubes, Elution Tubes
62124
454,00 €

The EZ1 DSP DNA Blood Kit is intended for in vitro diagnostic use.


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Eluate volume and PCR performance.|
Blood was collected from a healthy donor in BD K2E tubes and pooled before use. Genomic DNA was purified from twenty-four 350 μl aliquots in 4 runs of 6 replicates each using the EZ1 DSP DNA Blood system. The indicated amount of eluate was used in a 50 μl PCR with primers for a 1100 bp single-copy human gene fragment. +: positive control; -: negative control; M: low DNA mass ladder.|
Performance
Efficient purification of genomic DNA

Automated DNA purification provides high levels of reliability and standardization and helps to eliminate variation caused by handling errors. The EZ1 Advanced or the EZ1 Advanced XL in combination with the EZ1 DSP DNA Blood Kit enables efficient purification of genomic DNA from human whole blood. The amount of DNA purified depends on the white blood cell content (WBC) of each blood sample. The EZ1 DSP DNA Blood system provides high relative DNA yields independent of the donor’s WBC.

High-quality DNA for sensitive and specific analyses

Sensitive downstream applications demand the use of highly pure DNA for reliable results. DNA purified using the EZ1 DSP DNA Blood system is ready for immediate use in a broad range of sensitive diagnostic assays, such as real-time polymerase chain reaction (PCR) using CE-IVD-marked artus PCR Kits.

Unparalleled removal of inhibitors

Since PCR consists of multiple rounds of enzymatic reactions, it is more sensitive to impurities than single-step enzyme-catalyzed reactions. DNA purified using the EZ1 DSP DNA Blood system performs well in PCR even when large amounts of eluate are used, demonstrating the high purity of the DNA (see figure “Eluate volume and PCR performance”).

Principle

Automated sample preparation enables efficient, standardized purification of nucleic acids. The EZ1 Advanced and the EZ1 Advanced XL enable purification of nucleic acids using proven magnetic-particle technology for use in routine in vitro diagnostic applications. Reagent production is subjected to stringent quality control assuring reliable and robust performance. An easy-to-use instrument, prefilled and sealed reagent cartridges, and simple protocol selection and worktable setup help to prevent handling errors and allow the system to be operated by anyone — from the novice to the expert. Manual handling of potentially infectious samples is minimized, ensuring safety to the user and reliable processing of samples. Purified genomic DNA is ready to use in sensitive downstream diagnostic assays based on enzymatic amplification, such as PCR for genotyping.

Procedure

The EZ1 Advanced or the EZ1 Advanced XL in combination with the proven EZ1 DSP DNA Blood Kit enables purification of DNA from 1–6 samples (EZ1 Advanced) or 1-14 samples (EZ1 Advanced XL) of human whole blood. All processing steps are performed by the instrument, from piercing the reagent cartridge to elution of highly pure nucleic acids. The innovative design of the instrument means that the pipet tips function as separation chambers, increasing the efficiency of magnetic purification and eliminating the need for centrifugation steps. The streamlined procedure provides robust walkaway purification of nucleic acids with minimal hands-on time.

Applications

In combination with the EZ1 Advanced DSP DNA Blood Card and the EZ1 Advanced or the EZ1 Advanced XL DSP DNA Blood Card and the EZ1 Advanced XL, the EZ1 DSP DNA Blood Kit provides a fully automated procedure for purification of DNA from human whole blood. Genomic DNA purified using the EZ1 DSP DNA Blood Kit is ready to use in downstream diagnostic assays based on enzymatic amplification, such as human leukocyte antigen (HLA) and genetic testing.

Feature
Specifications
Applications PCR, real-time RT-PCR, genotyping
CE/FDA/IVD compatible CE/IVD
Elution volume 200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Whole blood
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein Genomic DNA
Sample amount 350 µl
Technology Magnetic particles
Time per run or per prep 15–20 minutes
Yield Varies on WBC count, please see handbook

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