For automated purification of high-quality DNA from 1–6 or 1–14 samples per run
  • Rapid purification of high-quality DNA
  • Standardized processing of up to 40 mg tissue samples
  • Flexible purification of a wide range of tissue types
  • Easy protocol setup with credit-card ease of use

The EZ1 DNA Tissue Kit contains all required reagents and labware for automated purification of DNA from up to 40 mg tissue samples using magnetic particle technology. The kit can be used with the EZ1 Advanced XL (1–14 samples per run), EZ1 Advanced (1–6 samples per run), and the BioRobot EZ1 (1–6 samples per run) instruments. Reagents are supplied in prefilled reagent cartridges, which ensures speed and convenience in loading the instrument. Protocols for automated purification of genomic DNA from a wide range sample types using the EZ1 DNA Tissue Kit are provided in the handbook. These include buccal cells, cultured cells, dried blood, and paraffin-embedded tissue sections.

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Product Cat. no. List price:
EZ1 DNA Tissue Kit (48)
For 48 preps: Reagent Cartridges (Tissue), Disposable Filter-Tips, Disposable Tip-Holders, Sample Tubes (2 ml), Elution Tubes (1.5 ml), Buffer G2, Proteinase K
953034
418,00 €
The EZ1 DNA Tissue Kit is intended for molecular biology applications. This product is not intended for the diagnosis, prevention, or treatment of a disease.
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Consistent PCR results.|
DNA was purified from various tissues using the EZ1 DNA Tissue procedure. [A] PCR of the bovine obesity single-copy gene (850 bp fragment) using EZ1 purified DNA from bovine tissues. [B] PCR of GAPDH (266 bp fragment) using EZ1 purified DNA from 6 mouse tails. A 2.5 µl aliquot (5%) of each PCR was visualized by agarose gel electrophoresis. M: 100 bp marker; -C: negative control.|
Performance
DNA purified using the EZ1 DNA Tissue Kit gives reproducible results in downstream applications, such as PCR (see figure "Consistent PCR results").
Principle

Automated purification with the EZ1 DNA Tissue Kit requires use of the EZ1 Advanced XL and the EZ1 Advanced XL DNA Tissue Card, or the EZ1 Advanced and the EZ1 Advanced DNA Tissue Card, or the BioRobot EZ1 and the EZ1 DNA Tissue Card. EZ1 cards are preprogrammed with protocols that guide the user through worktable setup and provide the instrument with commands for the purification procedure. Using silica-coated magnetic-particle technology, the easy-to-use instrument allows purification of 1–6 samples (EZ1 Advanced and BioRobot EZ1) or 1–14 samples (EZ1 Advanced XL) in a single run. Presealed reagent cartridges and the automatic sensor-controlled lock of the instrument door reduce the chances of environmental contamination. The automated procedure provides high repeatability and linear yields over a range of starting amounts.

Procedure

Tissues are first incubated in Buffer G2 containing proteinase K until lysed and then homogenized. DNA is then purified in one step on the instrument following the automated EZ1 DNA procedure. Genomic DNA is eluted in 200 µl, 100 µl, or 50 µl volumes, ready to use in all downstream applications, such as PCR.

Applications

The high-quality DNA obtained using the EZ1 DNA Tissue Kit is suited for use in many applications, such as:

Pharmacogenomic research
Oncology research
Genetic identity testing and forensics
STR, VNTR, RAPD, NASBA, SNP, and AFLP technologies
Feature
Specifications
Applications PCR, real-time RT-PCR, genotyping
Elution volume 200 µl
For automated processing BioRobot EZ1
Format Sample tube
Main sample type Tissue samples
Processing Automated
Purification of total RNA, miRNA, poly A+ mRNA, DNA or protein DNA
Sample amount 10–40 mg
Technology Magnetic particles
Time per run or per prep 40 minutes
Yield 9–28 µg

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Brochures & Guides
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Fully automated low- to medium-throughput purification of nucleic acids
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Kit Handbooks
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EZ1 DNA Tissue Kit
For automated purification of DNA from tissue and other samples using EZ1 instruments
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