Ordering Support Web
Simplify kit selection with our helpful selection guides

 

  • Main Image Navi
Not sure which product to choose? Our helpful selection guides make decision making a lot easier!
Choose from the following:
Small-scale plasmid purification — 96-well format
Small-scale plasmid purification kits
Large-scale plasmid purification kits


Small-scale plasmid purification — 96-well format
DNA grade  
                                                                             Transfection grade
 Molecular biology grade                                                                            Sequencing grade
Speed  Standard Fast Fast Standard
Kit name QIAGEN Plasmid Plus 96 Miniprep and BioRobot Kits QIAprep 96 Plus Miniprep and BioRobot Kits QIAprep 96 Turbo Miniprep and BioRobot Kits  R.E.A.L. Prep 96 Plasmid Kit
Binding capacity <50 µg <50 µg <20 µg <30 µg
Culture volume (3–4 x 109 cells/ml) 1.25 –5 ml 1.25 –5 ml 1–5 ml 2.5 ml BAC/PAC/P1, 1.3 ml plasmid, 1.3–2.5 ml cosmid
Yield 15–50 µg 15–50 µg 4–20 µg 1 µg BAC/PAC/P1
Elution volume 80 µl (100–120 µl for a 5 ml culture) 80 µl (100–120 µl for a 5 ml culture) 100 µl 22 µl BAC/PAC/P1, 50–250 µl plasmid, 50 µl cosmid
Procedure QIAvac 96, centrifuge, BioRobot Universal System QIAvac 96, centrifuge, BioRobot Universal System QIAvac 96, centrifuge, BioRobot Universal System QIAvac 96, centrifuge
Time 50 min 40 min 45 min 70 min

Small-scale plasmid purification kits
DNA grade  Transfection grade  Molecular biology grade                                                                            Sequencing grade
Speed Fast Fast Ultrafast
Kit name QIAGEN Plasmid Mini Kit QIAprep Spin Miniprep Kit Quicklyse Miniprep Kit
Binding capacity <20 µg <50 µg <15 µg
Culture volume (3–4 x 109 cells/ml) 3 ml 1–5 ml 1–3 ml
Yield 20 µg 5–50 µg 8–15 µg
Elution volume Variable, 50 µl recommended 50 µl 50 µl
Procedure Centrifuge, QIAcube QIAvac 24 Plus, centrifuge, QIAcube Centrifuge
Time 80 min 30 min 9 min

Large-scale plasmid purification kits
DNA grade    Endotoxin-free                                                                                                                                         Transfection grade                                        Molecular biology grade
Speed Standard Standard Fast
Ultrafast Ultrafast Standard Ultrafast
Kit name   EndoFree Plasmid Kits QIAGEN Plasmid Kits QIAfilter Plasmid Kits QIAGEN Plasmid Plus Kits HiSpeed Plasmid Kits QIAGEN Large-Construct Kit CompactPrep Plasmid Kits
Culture volume (3–4 x 109 cells/ml) Midi   _
 25 ml, 100 ml
25 ml, 50–100 ml
20–25 ml, 25–35 ml (hy), 50 ml 50 ml, 150 ml
500 ml 25 ml, 50 ml
Maxi 100 ml, 250 ml
 100 ml, 500 ml
100 ml, 250 ml
80–100 ml, 100–130 ml (hy), 200 ml
150 ml, 250 ml
 100 ml, 250 ml
Mega 500 ml/1.5 g, 2.5 l / 7.5g
500 ml/1.5 g, 2.5 l/ 7.5g
500 ml/1.5 g, 2.5 l/7.5g
500 ml
500 ml only rec for hc
Giga 2.5 l/7.5 g, not rec. for lc
2.5 l/7.5 g, 5 l/15 g
2.5 l/7.5 g, not rec. for lc
2.5 l
2.5 l only rec for hc
Yield Midi  –
75–100 µg, 20–100 µg
75–100 µg, 20–100 µg
100–200 µg, 150–250 µg (hy), 30–100 µg
100–200 µg, 30–100 µg
Up to 50 µg BAC/PAC/P1 DNA, up to 200 µg cosmid DNA  100–200 µg, 30–100 µg
Maxi 300–500 µg, 50–250 µg
100–500 µg, 20–250 µg
300–500 µg, 50–250 µg
750 µg, 1 mg (hy), 50–250 µg
300–750 µg, 50–250 µg
350–750 µg, 50–250 µg
Mega 1.5–2.5 mg, 0.5–2.5 mg
1.5–2.5 mg, 0.5–2.5 mg
1.5–2.5 mg, 0.5–2.5 mg
Up to 2.5 mg
1.5–2.5 mg, endotoxin-free <0.1 EU/µg DNA
Giga 7.5–10 mg
7.5–10 mg, 1–5 mg
7.5–10 mg
Up to 10 mg
7–10 mg, endotoxin-free <0.1 EU/µg DNA
Time Midi  –
150 min
110 min
20 min
<45 min
280 min <20 min
Maxi 150 min
160 min
120 min
20 min
<60 min
<20 min
Mega 220 min
220 min
190 min
40 min
50 min
Giga 310 min
320 min
280 min
50 min
50 min
Procedure Lysate clearing by precipitator or vacuum, gravity-flow Gravity-flow Lysate clearing by precipitator or vacuum, gravity-flow Vacuum and centriguge Gravity-flow and precipitator Centrifuge Centrifuge and vacuum
lc = low copy, hc = high copy, hy = high yield; Sample size/volume: e.g., 500 ml / 1.5 g = pellet wet weight; rec = recommended