Quantification and Profiling of microRNAs: Challenges and Strategies


miRNA quantification presents many challenges, due to the small size of miRNAs, their 3' heterogeneity, and the low amounts of miRNA present in samples. The new, next-generation miScript PCR System is a robust and accurate qRT-PCR- based system for transcriptomewide miRNA profiling in mammalian cells. A key feature of the miScript PCR System is its use of a universal reverse transcription reaction, facilitating quantification of all miRNAs as well as their target mRNAs using the same cDNA preparation.

This 1-hour webinar will cover:

  • Latest strategies for miRNA quantification
  • Understanding critical parameters: specificity and sensitivity
  • miRNA profiling strategy
  • Quantifying precursor and mature miRNA to understand gene regulation

Constanze Kindler, Ph.D.

Constanze Kindler
 

Dr. Constanze Kindler joined QIAGEN in Hilden, Germany in 1993, where she held various positions in Sales and Global Marketing. She received her Ph.D in Molecular Biology from the University of Münster in 1993. Currently Dr. Kindler is Senior Global Product Manager Sample Technologies with a focus on liquid biopsy applications.