cDNA Synthesis and SYBR Green- or Probe-Based (RT-)PCR

We offer a complete portfolio for quantitative PCR and one-step or two-step qRT-PCR using SYBR Green- or probe-based chemistry. SYBR Green kits provide a ready-to-use format for detection of any target; you can simply use our dedicated GeneGlobe resources to design your own specific primers. Alternatively, solutions for probe-based detection require labeled probes (e.g., TaqMan®, Scorpions, etc.) to be designed, providing an additional level of specificity during detection. Probe-based procedures also allow for multiplexing, so you can make more efficient use of limited resources, and also include internal controls or reference genes to improve accuracy and confidence in your data.

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For isolation and purification of intracellular RNA from blood stabilized in PAXgene Blood RNA Tubes
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For fixation and stabilization of human tissue specimens (max. 4 x 15 x 15 mm in size)
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For collection, RNA stabilization, transport, and storage of tissues
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For stabilization and purification of up to 100 µg total RNA from tissues
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For fully automated sample prep using QIAGEN spin-column kits
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Instrument for automated mid- to high-throughput nucleic acid purification in 96-well format
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For accelerated DNA, RNA, and protein quantification and quality control
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For fully integrated automation of complete workflows, from sample preparation to assay setup
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For isolation and purification of intracellular RNA from blood stabilized in PAXgene Blood RNA Tubes
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For purification of miRNA and total RNA from whole blood
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For purification of total RNA from tissues fixed and stabilized in PAXgene Tissue Containers
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For simultaneous purification of genomic DNA and total RNA, including miRNA, from cells and difficult-to-lyse tissue
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For purification of cell-free total RNA, including miRNA, from animal and human plasma and serum
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For purification of up to 45 µg total RNA from cells/tissues using gDNA Eliminator columns
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For purification of up to 100 µg total RNA from cells/tissues using gDNA Eliminator columns
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For purification of total RNA from all types of tissue using gDNA Eliminator Solution
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For purification of up to 100 µg total RNA from fiber-rich tissues
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For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections
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For simultaneous purification of genomic DNA and total RNA (including small RNAs) from formalin-fixed, paraffin-embedded tissue sections
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For stabilization and purification of up to 100 µg total RNA from tissues
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For rapid, high-precision automated PCR setup
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For whole transcriptome amplification of total RNA or mRNA from single cells
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For fast cDNA synthesis and reproducible real-time two-step RT-PCR
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For fast cDNA synthesis enabling sensitive real-time two-step RT-PCR for gene expression analysis
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For parallel whole genome and whole transcriptome amplification from cells and limited samples
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For outstanding performance in real-time PCR
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For fully integrated automation of complete workflows, from sample preparation to assay setup
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For one-step qRT-PCR using SYBR Green I for gene expression analysis
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For one-step qRT-PCR using sequence-specific probes for gene expression analysis
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For unparalleled results using SYBR Green-based qPCR
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For highly sensitive, specific, and ultrafast, probe-based real-time PCR
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For SYBR® Green-based expression analysis of individual genes using bioinformatically-verified assays
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For fast, multiplex, real-time PCR and two-step qRT-PCR using sequence-specific probes
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For fast, multiplex, one-step qRT-PCR using sequence-specific probes for gene expression analysis
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For modeling, analyzing, and understanding complex 'omics data
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For analyzing and interpreting real-time PCR or NGS data
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