RNA Quality Control – Comparing Different RNA Quality Indicator Numbers

RNA quantification and QC are critical to successful downstream RT-PCR, microarray and RNAseq analyses, as the results are heavily impacted by the purity and integrity of the input RNA. Conventional methods for assessing RNA integrity such as denaturing gel electrophoresis, have been replaced in recent years by more convenient microfluidics- or capillary-based technologies. These new automated methods are advantageous, requiring less sample input while providing standardized processing with an objective RNA integrity assessment. Different companies have established their own RNA quality indicator numbers, based on proprietary algorithms considering various electrophoretic features of the analyzed samples.

In this webinar we will present a comparison of two different RNA quality indicator numbers for RNA purified from three eukaryotic cell lines and subsequently degraded by heat, RNase or UV light. We will also show the experimental assessment of the corresponding mRNA integrity by quantitative RT-PCR, to better compare and evaluate the two quality indicators. In addition, we will discuss the RNA integrity assessments for biochemically and biophysically degraded RNA.

Pierre-Henri Ferdinand, Ph.D., Associate Global Product Manager, QIAGEN

Dr. Pierre-Henri Ferdinand joined QIAGEN in Hilden, Germany in 2014 as Associate Global Product Manager responsible for the QIAxcel Advanced platform. Pierre-Henri received his Ph.D. in Molecular Microbiology and Biotechnology in 2013 from the Aix-Marseille University in France. In 2014 he completed his M.Sc. in Business Administration and Bio-entrepreneurship from the Copenhagen Business School in Denmark.
Conny Unger, M.Sc.

Conny Unger holds a Bachelor of Biomedicine degree from the Julius-Maximillans University in Würzburg, Germany. She also studied at the University Duisburg-Essen to pursue a Master in Medical Biology. She came to QIAGEN in 2014 for a 5-month internship and wrote her Master's thesis on RNA degradation and the comparison of RNA integrity measurement systems.