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Cat. No. / ID: 205411
✓ 24/7 automatic processing of online orders
✓ Knowledgeable and professional Product & Technical Support
✓ Fast and reliable (re)-ordering
Note: The QuantiNova Reverse Transcription Kit (50) 205411 and (200) 205413 are temporarily unavailable. We recommend using the QuantiTect Reverse Transcription Kit (50) 205311, (200) 205313 or (400) 205314. It is fully compatible with all QuantiNova Reverse Transcription Kit applications, including two-step RT-qPCR using QuantiNova PCR kits and/or QuantiNova LNA assays or panels. It also offers integrated genomic DNA removal, ultrafast 20-minute protocol, random primers and high transcription efficiency. However, the total RNA input is limited to 1 µg, and this kit does not provide an Internal Control RNA.
Find out more about the QuantiTect Reverse Transcription Kit. If you need further assistance or have any questions about the change, please do not hesitate to contact our team of experts via your local QIAGEN representatives.
The QuantiNova Reverse Transcription Kit provides a fast and convenient procedure for cDNA synthesis with integrated genomic DNA removal and an additional internal control. Genomic DNA contamination in RNA samples is effectively eliminated by the gDNA Removal Mix. The QuantiNova Reverse Transcription Kit provides everything you need for fast and efficient reverse transcription. The synthesized cDNA is optimized for use in real-time PCR, allowing reliable quantification of targets from all regions of mRNA transcripts.
The internal control RNA can be used to test successful reverse transcription and amplification using the QuantiNova IC Probe Assay (Max label, cat. no. 205813) or QuantiNova IC Probe Assay Red 650 (Cy5 analog label, cat. no. 205824) for probe based detection. For SYBR Green based detection please order via our GeneGlobe platform: QuantiNova IC SYBR Green Assay (Available under the name HS_QIC_2467742 QuantiNova LNA PCR Reference Assay and the GeneGlobe ID: SBH1218551).
Component | Benefits |
---|---|
gDNA Removal Mix | Detection of RNA only in real-time RT-PCR |
Internal Control RNA | Verification of successful RT-PCR performance |
QuantiNova Reverse Transcriptase | Use of a wide range of RNA amounts (10 pg – 5 μg RNA) High sensitivity |
QuantiNova RT buffer system | Read-through of difficult templates |
RT Primer Mix | cDNA synthesis from all regions of transcripts, even from 5' regions |
Genomic DNA removal and cDNA synthesis take only 20 minutes with the QuantiNova Reverse Transcription Kit. The kit includes everything you need for fast cDNA synthesis.
Purified RNA is briefly incubated in gDNA Removal Mix to effectively remove contaminating genomic DNA. In contrast to other methods, the RNA sample is then used directly for reverse transcription in combination with a master mix prepared from the Reverse Transcription Mix plus Reverse Transcription Enzyme. With the QuantiNova Reverse Transcriptase, RNA can be transcribed at low temperatures, even through complex secondary structure. This ensures that the RNA will stay intact as the reaction takes place at 42°C and is then inactivated at 85°C. Additional steps for RNA denaturation or RNase H digestion are not necessary.
The internal control (IC) RNA can be transcribed simultaneously with the RNA sample and is intended to report the presence of inhibitors, instrument or chemistry failures or errors in assay setup.
The QuantiNova IC Probe Assay (Max label, cat. no. 205813) or QuantiNova IC Probe Assay Red 650 (Cy5 analog label, cat. no. 205824) are used for probe based detection procedures of the Internal Control RNA or DNA provided in the QuantiNova Reverse Transcription Kit, the QuantiNova Probe RT-PCR Kit (one-step RT-PCR) or the QuantiNova Pathogen +IC Kit.
The QuantiNova IC SYBR Green Assay (cat. no. SBH1218551) is used in conjunction with the Internal Control RNA provided in the QuantiNova Reverse Transcription Kit (two-step RT-PCR), the QuantiNova SYBR Green RT-PCR Kit (one-step RT-PCR) and the QIAcuity OneStep Eva Green Kit. It permits assessment of reverse transcription/amplification performance using the QuantiNova SYBR Green PCR Kit (two-step RT-PCR), the QuantiNova SYBR Green RT-PCR Kit (one-step RT-PCR), the QIAcuity OneStep Eva Green Kit or the QuantiNova LNA PCR Custom Panels.
For a streamlined workflow, we recommend combining QuantiNova Kits with our predesigned QuantiNova real-time PCR assays or panels. These offer precise quantification of mRNA or long non-coding RNA transcripts, regardless of abundance. Choose from a wide range of predesigned human, mouse and rat primer sets, or customize your own assays and panels using our advanced design tools.
Our QuantiNova LNA PCR and QuantiNova LNA Probe PCR Assays utilize LNA technology for enhanced sensitivity, enabling unbiased gene expression profiles and faster scientific insights.
Note: The QuantiNova IC SYBR Green Assay was formerly available as QuantiTect Primer Assay for SYBR based detection of IC RNA (cat. no. QT02589307).